Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_21355 and AO356_21360 are separated by 75 nucleotidesAO356_21360 and AO356_21365 are separated by 181 nucleotidesAO356_21365 and AO356_21370 are separated by 28 nucleotidesAO356_21370 and AO356_21375 are separated by 85 nucleotides AO356_21355: AO356_21355 - tRNA-Ala, at 4,789,207 to 4,789,282 _21355 AO356_21360: AO356_21360 - tRNA-Glu, at 4,789,358 to 4,789,433 _21360 AO356_21365: AO356_21365 - TetR family transcriptional regulator, at 4,789,615 to 4,790,154 _21365 AO356_21370: AO356_21370 - hydrolase, at 4,790,183 to 4,791,016 _21370 AO356_21375: AO356_21375 - 4-hydroxybenzoyl-CoA thioesterase, at 4,791,102 to 4,791,551 _21375
Group Condition AO356_21355 AO356_21360 AO356_21365 AO356_21370 AO356_21375
carbon source Carbon source D-Mannitol 2.5 mM N.D. N.D. -0.2 -1.4 +0.1
motility outer cut, LB soft agar motility assay N.D. N.D. -0.3 -1.0 -0.1
survival 4C survival; 1152 hrs N.D. N.D. -0.5 -0.5 -0.4
stress acetate 40 mM N.D. N.D. -0.0 -0.6 -0.7
stress Cholin acetate 40 mM N.D. N.D. -0.4 -0.6 -0.3
anaerobic Nitrate reduction with yeast extract N.D. N.D. +0.5 -1.5 -0.2
stress Doxycycline hyclate 0.0001 mg/ml N.D. N.D. -0.6 -0.5 +0.0
stress Nalidixic 0.004 mg/ml N.D. N.D. -0.4 -0.8 +0.3
carbon source Carbon source D-Glucose 10 mM N.D. N.D. -0.3 -0.7 +0.2
stress Sisomicin 0.001 mg/ml N.D. N.D. +0.3 -1.0 -0.1
survival 4C survival; 192 hrs N.D. N.D. -0.3 +0.1 -0.6
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. N.D. -1.1 +0.4 +0.1
carbon source Carbon source Sucrose 5 mM N.D. N.D. -0.3 -0.5 +0.2
stress copper (II) chloride 2.5 mM N.D. N.D. -0.3 -0.6 +0.3
lb LB N.D. N.D. -0.2 -0.7 +0.4
carbon source Putrescine (C) N.D. N.D. -0.7 +0.2 +0.0
carbon source Carbon source D,L-Lactate 10 mM N.D. N.D. -0.1 -0.6 +0.3
stress Chlorite 0.04 mM N.D. N.D. +0.4 -0.9 +0.1
stress Cisplatin 0.0032 mg/ml N.D. N.D. +0.2 -0.8 +0.4
carbon source L-Arginine (C) N.D. N.D. +0.5 -0.5 -0.1
carbon source L-Leucine (C) N.D. N.D. +0.5 -0.2 -0.3
stress Choline chloride 500 mM N.D. N.D. +0.7 +0.0 -0.3
stress KB-half, Pseudo5 supernatant N.D. N.D. +0.6 -0.4 +0.2
carbon source L-Histidine (C) N.D. N.D. +0.7 -0.2 +0.1
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. N.D. +1.1 -0.5 +0.3
stress Nickel (II) chloride 0.625 mM N.D. N.D. +0.3 +0.6 +0.1
carbon source Fumarate (C) N.D. N.D. +0.3 +0.6 +0.1
carbon source Fumarate (C) N.D. N.D. +0.6 +0.4 +0.3
stress Nickel (II) chloride 0.625 mM N.D. N.D. +0.7 +0.6 +0.0
stress Chlorite 0.08 mM N.D. N.D. +0.8 +0.6 +0.3
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