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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_21245 and AO356_21250 are separated by 12 nucleotides
AO356_21250 and AO356_21255 are separated by 273 nucleotides
AO356_21255 and AO356_21260 are separated by 180 nucleotides
AO356_21260 and AO356_21265 are separated by 83 nucleotides
AO356_21245: AO356_21245 - cytosine deaminase, at 4,767,732 to 4,768,979
_21245
AO356_21250: AO356_21250 - cytosine permease, at 4,768,992 to 4,770,263
_21250
AO356_21255: AO356_21255 - alpha/beta hydrolase, at 4,770,537 to 4,771,367
_21255
AO356_21260: AO356_21260 - 6-carboxy-5,6,7,8-tetrahydropterin synthase, at 4,771,548 to 4,771,904
_21260
AO356_21265: AO356_21265 - hypothetical protein, at 4,771,988 to 4,773,067
_21265
Group
Condition
AO356
_21245
AO356
_21250
AO356
_21255
AO356
_21260
AO356
_21265
stress
Chlorite 0.04 mM
-0.1
-0.0
-0.0
-3.5
+0.2
motility
outer cut, LB soft agar motility assay
-0.6
-0.2
-0.2
-1.2
-0.3
carbon source
L-Arginine (C)
-0.2
-0.1
+0.0
-1.5
-0.2
stress
methylglyoxal 0.016 vol%
-0.3
-0.1
-0.1
-1.2
-0.3
anaerobic
Nitrate reduction with yeast extract
-1.4
-0.5
-0.4
+0.4
-0.1
carbon source
NAG (C)
+0.5
-0.0
-0.2
-2.0
-0.2
carbon source
L-Proline (C)
-0.0
+0.1
-0.1
-2.0
+0.1
nitrogen source
Cytosine (N)
-1.6
-0.2
+0.1
-0.1
+0.0
stress
Cobalt chloride 0.08 mM
-0.4
+0.1
-0.1
-1.2
-0.2
carbon source
Fumarate (C)
-0.3
+0.0
-0.2
-1.0
-0.3
stress
Chlorite 0.08 mM
-0.2
-0.1
+0.0
-1.4
-0.0
carbon source
Carbon source Sucrose 2.5 mM
-0.4
+0.1
-0.2
-0.8
-0.2
carbon source
D-Galactose (C)
-0.2
+0.1
+0.1
-1.3
-0.2
stress
Sisomicin 0.001 mg/ml
+0.1
-0.0
+0.4
-2.0
+0.2
carbon source
D-Gluconic Acid (C)
-0.0
+0.1
-0.1
-1.2
-0.0
carbon source
Carbon source Sucrose 5 mM
-0.0
-0.0
+0.2
-1.4
+0.0
stress
Choline chloride 300 mM
+0.2
-0.3
-0.1
-0.7
-0.2
stress
nitrate 200 mM
+0.0
-0.1
+0.2
-1.5
+0.3
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.8
-0.0
-0.1
-0.4
+0.3
carbon source
L-Histidine (C)
+0.4
+0.2
+0.1
-1.9
+0.1
motility
outer cut, LB soft agar motility assay
+0.9
+0.4
+0.1
-2.7
+0.3
stress
Lomefloxacin 0.0005 mM
-0.7
+0.2
+0.3
-0.5
-0.3
carbon source
Glycerol (C)
-0.2
+0.1
+0.1
-0.9
+0.4
stress
Nickel (II) chloride 0.625 mM
-0.3
-0.1
-0.2
+0.7
-0.6
stress
Cholin acetate 40 mM
-0.9
-0.1
+0.1
+0.3
+0.2
stress
copper (II) chloride 2.5 mM
-0.5
+0.0
-0.6
+1.4
-0.5
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.6
-0.2
-0.0
-0.7
+0.5
stress
Choline chloride 500 mM
+0.1
-0.1
+0.2
+1.0
-0.3
motility
outer cut, LB soft agar motility assay
-0.4
+0.1
-0.0
+1.1
+0.2
motility
outer cut, LB soft agar motility assay
+0.4
+0.2
+0.2
+0.7
+0.3
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