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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_20965 and AO356_20970 are separated by 89 nucleotides
AO356_20970 and AO356_20975 are separated by 6 nucleotides
AO356_20975 and AO356_20980 are separated by 168 nucleotides
AO356_20980 and AO356_20985 are separated by 670 nucleotides
AO356_20965: AO356_20965 - glucose 1-dehydrogenase, at 4,667,283 to 4,668,011
_20965
AO356_20970: AO356_20970 - TetR family transcriptional regulator, at 4,668,101 to 4,668,670
_20970
AO356_20975: AO356_20975 - hypothetical protein, at 4,668,677 to 4,668,958
_20975
AO356_20980: AO356_20980 - transporter, at 4,669,127 to 4,670,017
_20980
AO356_20985: AO356_20985 - 16S ribosomal RNA, at 4,670,688 to 4,672,233
_20985
Group
Condition
AO356
_20965
AO356
_20970
AO356
_20975
AO356
_20980
AO356
_20985
stress
Benzalkonium Chloride 0.016 mg/ml
+0.3
-0.6
-1.1
+0.1
N.D.
stress
KB-half, Pseudo5 supernatant
-0.2
-0.3
-0.5
-0.3
N.D.
survival
4C survival; 192 hrs
-0.0
-0.3
-0.8
+0.0
N.D.
motility
outer cut, LB soft agar motility assay
+0.1
+0.1
-1.3
-0.0
N.D.
anaerobic
Nitrate reduction with yeast extract
-0.5
-0.5
-0.1
+0.1
N.D.
pH
Minimal media pH9
-0.0
-0.1
-0.5
-0.1
N.D.
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.2
+0.1
-0.4
-0.1
N.D.
stress
Cholin acetate 20 mM
+0.1
-0.5
-0.2
+0.1
N.D.
motility
outer cut, LB soft agar motility assay
-0.6
+0.3
-0.5
+0.2
N.D.
survival
4C survival; 1152 hrs
-0.1
+0.2
-0.6
+0.0
N.D.
nitrogen source
D-Alanine (N)
+0.1
-0.3
-0.4
+0.0
N.D.
resistance
20 mM putrescine
-0.2
+0.2
-0.5
-0.0
N.D.
stress
1-ethyl-3-methylimidazolium acetate 5 mM
+0.2
-0.8
+0.1
+0.0
N.D.
survival
4C survival; 360 hrs
+0.1
+0.1
-0.6
-0.1
N.D.
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.1
+0.2
-0.4
-0.0
N.D.
carbon source
D-Alanine (C)
+0.0
+0.2
-0.5
-0.0
N.D.
carbon source
L-Histidine (C)
+0.0
+0.0
+0.3
-0.6
N.D.
stress
1-ethyl-3-methylimidazolium acetate 20 mM
+0.3
-0.3
-0.2
+0.1
N.D.
stress
copper (II) chloride 2.5 mM
+0.2
-0.5
-0.1
+0.3
N.D.
carbon source
Ethanol (C)
-0.0
-0.4
+0.3
+0.1
N.D.
carbon source
L-Malic (C)
-0.1
-0.4
+0.4
+0.1
N.D.
carbon source
D-Ribose (C)
+0.1
-0.2
+0.3
-0.2
N.D.
motility
outer cut, LB soft agar motility assay
+0.3
+0.6
-0.7
-0.0
N.D.
carbon source
D-Xylose (C)
+0.2
-0.1
+0.3
-0.2
N.D.
carbon source
Carbon source Sucrose 5 mM
+0.1
+0.4
-0.2
-0.0
N.D.
carbon source
Fumarate (C)
+0.3
-0.1
+0.3
+0.1
N.D.
carbon source
L-Arabinose (C)
+0.2
+0.1
+0.5
-0.1
N.D.
carbon source
Carbon source Sucrose 10 mM
-0.1
+0.3
+0.5
+0.1
N.D.
nitrogen source
Adenosine (N)
+0.2
+0.4
+0.2
-0.0
N.D.
carbon source
Fumarate (C)
+0.1
+0.1
+0.5
+0.1
N.D.
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