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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_20840 and AO356_20845 are separated by 281 nucleotides
AO356_20845 and AO356_20850 overlap by 1 nucleotides
AO356_20850 and AO356_20855 are separated by 5 nucleotides
AO356_20855 and AO356_20860 are separated by 283 nucleotides
AO356_20840: AO356_20840 - aminodeoxychorismate synthase component I, at 4,642,363 to 4,643,706
_20840
AO356_20845: AO356_20845 - alpha-L-glutamate ligase, at 4,643,988 to 4,644,974
_20845
AO356_20850: AO356_20850 - hypothetical protein, at 4,644,974 to 4,646,506
_20850
AO356_20855: AO356_20855 - ATP-dependent zinc protease, at 4,646,512 to 4,647,048
_20855
AO356_20860: AO356_20860 - GntR family transcriptional regulator, at 4,647,332 to 4,648,045
_20860
Group
Condition
AO356
_20840
AO356
_20845
AO356
_20850
AO356
_20855
AO356
_20860
stress
Benzalkonium Chloride 0.016 mg/ml
+0.4
-4.7
-4.6
-2.7
-0.0
motility
outer cut, LB soft agar motility assay
-0.0
-1.6
-1.6
-2.3
-1.2
stress
Doxycycline hyclate 0.0001 mg/ml
-0.1
-2.3
-2.3
-1.9
+0.3
stress
Lomefloxacin 0.0005 mM
-0.3
-1.5
-3.1
-0.4
+0.3
stress
Benzalkonium Chloride 0.008 mg/ml
+0.3
-1.6
-1.9
-1.4
+0.0
stress
Benzethonium chloride 0.025 mM
-0.4
-1.6
-1.9
-0.5
+0.1
motility
outer cut, LB soft agar motility assay
+0.4
-1.0
-2.0
-1.0
-0.7
stress
Cholin acetate 40 mM
-0.3
-1.3
-1.6
-0.9
-0.1
stress
Nalidixic 0.004 mg/ml
-0.4
-1.5
-1.4
-0.9
+0.1
motility
outer cut, LB soft agar motility assay
-0.0
-1.2
-1.9
-1.4
+0.5
stress
1-ethyl-3-methylimidazolium lysine 10 mM
-0.3
-1.0
-0.9
-1.2
-0.3
stress
Rifampicin 0.0024 mg/ml
-0.2
-1.3
-1.1
-0.9
+0.1
pH
Minimal media pH9
+0.0
-0.7
-1.9
-0.6
-0.1
pH
Minimal media pH6
-0.2
-1.3
-1.4
-0.4
+0.1
motility
outer cut, LB soft agar motility assay
+0.1
-1.7
-0.8
-0.4
-0.1
carbon source
L-Asparagine (C)
-0.2
-0.8
-1.3
-0.6
+0.0
stress
methylglyoxal 0.016 vol%
-0.2
-1.4
-0.7
-0.5
-0.1
stress
1-ethyl-3-methylimidazolium acetate 10 mM
-0.7
-0.5
-1.1
-0.6
-0.0
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.2
-0.5
-1.7
-0.6
-0.2
stress
Cholin acetate 20 mM
-0.2
-0.8
-1.1
-0.4
-0.0
stress
Cephalothin 2 mg/ml
-0.5
-1.1
-0.7
-0.3
+0.1
stress
Tetracycline 0.0001 mg/ml
+0.1
-0.8
-1.0
-0.8
+0.2
stress
Nickel (II) chloride 0.625 mM
+0.4
-0.8
-1.0
-0.7
+0.1
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.5
+0.4
-0.4
-1.0
+0.2
stress
Chlorite 0.08 mM
+0.5
-0.6
-1.2
+0.2
-0.2
carbon source
L-Isoleucine (C)
-0.0
+0.8
+0.8
+0.5
-1.0
carbon source
L-Lysine (C)
+0.2
+0.8
+1.0
+1.1
+0.1
carbon source
L-Lysine (C)
-0.2
+1.2
+1.2
+0.8
+0.4
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.3
+1.3
+1.4
+1.1
+0.1
stress
Sisomicin 0.001 mg/ml
+0.8
+2.3
+1.9
+1.5
+0.7
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