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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_20455 and AO356_20460 are separated by 222 nucleotides
AO356_20460 and AO356_20465 are separated by 0 nucleotides
AO356_20465 and AO356_20470 are separated by 43 nucleotides
AO356_20470 and AO356_20475 are separated by 102 nucleotides
AO356_20455: AO356_20455 - toprim domain protein, at 4,566,743 to 4,569,310
_20455
AO356_20460: AO356_20460 - hypothetical protein, at 4,569,533 to 4,569,772
_20460
AO356_20465: AO356_20465 - transcriptional regulator, at 4,569,773 to 4,570,060
_20465
AO356_20470: AO356_20470 - hypothetical protein, at 4,570,104 to 4,570,295
_20470
AO356_20475: AO356_20475 - hypothetical protein, at 4,570,398 to 4,570,745
_20475
Group
Condition
AO356
_20455
AO356
_20460
AO356
_20465
AO356
_20470
AO356
_20475
anaerobic
Nitrate reduction with yeast extract
+0.0
-0.8
-0.4
-1.2
-5.6
pH
Minimal media pH9
+0.2
+0.0
+0.1
-0.2
-6.3
motility
outer cut, LB soft agar motility assay
+0.2
+0.8
-1.2
-0.5
-5.2
survival
4C survival; 192 hrs
-0.3
-1.0
-0.1
-0.5
-3.9
survival
4C survival; 360 hrs
-0.3
-0.1
-0.7
-0.8
-3.7
stress
copper (II) chloride 2.5 mM
-0.0
-0.5
-0.4
-0.4
-4.1
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.1
+0.1
-0.7
-1.9
-2.7
nitrogen source
Adenosine (N)
-0.1
-0.3
-0.1
-1.2
-3.4
stress
Choline chloride 300 mM
-0.3
-0.6
-0.0
-0.2
-4.0
stress
Sisomicin 0.001 mg/ml
-0.1
-0.0
+0.2
-0.2
-4.7
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.0
+0.0
-0.7
-0.2
-3.7
survival
4C survival; 1152 hrs
-0.0
+0.1
-0.5
-1.9
-2.3
survival
4C survival; 672 hrs
-0.1
-0.2
-0.6
-1.1
-2.5
stress
nitrate 200 mM
+0.1
-0.1
-0.0
+0.4
-4.7
carbon source
Carbon source Sucrose 5 mM
+0.2
-0.2
-0.1
-1.3
-2.9
pH
Minimal media pH6
-0.1
-0.4
+0.1
-1.6
-2.2
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.1
-0.3
-0.4
-1.9
-1.5
nitrogen source
Urea (N)
-0.1
-0.1
-0.3
-0.7
-2.8
stress
acetate 40 mM
+0.1
-0.2
+0.1
-1.1
-2.9
motility
outer cut, LB soft agar motility assay
+0.0
+0.0
-0.2
-1.3
-2.6
resistance
20 mM putrescine
-0.1
-0.1
-0.3
-0.7
-2.9
carbon source
D-Glucosamine Hydrochloride (C)
+0.2
-0.2
+0.0
-0.2
-3.7
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.1
-0.3
-0.4
-1.1
-2.0
stress
Benzethonium chloride 0.025 mM
+0.1
-0.0
-0.3
+0.0
-3.4
carbon source
Carbon source D-Mannitol 5 mM
+0.2
+0.3
+0.2
-0.8
-3.2
stress
Benzalkonium Chloride 0.016 mg/ml
+0.4
+0.6
-0.4
-0.8
-3.1
carbon source
Carbon source Sucrose 2.5 mM
+0.0
+0.3
-0.1
-1.2
-2.2
carbon source
Carbon source Sucrose 10 mM
+0.1
+0.3
-0.1
-0.8
-2.8
carbon source
NAG (C)
-0.2
+0.3
+0.2
-0.3
-3.2
stress
Lomefloxacin 0.0005 mM
+0.2
-0.5
+1.1
-0.1
+2.9
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