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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_20450 and AO356_20455 are separated by 63 nucleotides
AO356_20455 and AO356_20460 are separated by 222 nucleotides
AO356_20460 and AO356_20465 are separated by 0 nucleotides
AO356_20465 and AO356_20470 are separated by 43 nucleotides
AO356_20450: AO356_20450 - hypothetical protein, at 4,566,341 to 4,566,679
_20450
AO356_20455: AO356_20455 - toprim domain protein, at 4,566,743 to 4,569,310
_20455
AO356_20460: AO356_20460 - hypothetical protein, at 4,569,533 to 4,569,772
_20460
AO356_20465: AO356_20465 - transcriptional regulator, at 4,569,773 to 4,570,060
_20465
AO356_20470: AO356_20470 - hypothetical protein, at 4,570,104 to 4,570,295
_20470
Group
Condition
AO356
_20450
AO356
_20455
AO356
_20460
AO356
_20465
AO356
_20470
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.5
-0.1
-0.3
-0.4
-1.9
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.3
-0.1
+0.1
-0.7
-1.9
survival
4C survival; 1152 hrs
-0.2
-0.0
+0.1
-0.5
-1.9
survival
4C survival; 672 hrs
-0.5
-0.1
-0.2
-0.6
-1.1
survival
4C survival; 360 hrs
-0.5
-0.3
-0.1
-0.7
-0.8
anaerobic
Nitrate reduction with yeast extract
+0.2
+0.0
-0.8
-0.4
-1.2
carbon source
Carbon source Sucrose 10 mM
+0.2
-0.1
-0.5
+0.2
-1.9
stress
Nickel (II) chloride 0.625 mM
-0.0
-0.0
-0.4
-0.1
-1.4
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.2
-0.1
-0.3
-0.4
-1.1
lb
LB
-0.4
-0.1
-0.6
-0.2
-0.7
survival
4C survival; 192 hrs
-0.2
-0.3
-1.0
-0.1
-0.5
stress
Cisplatin 0.0032 mg/ml
-0.3
+0.2
-0.1
+0.4
-2.0
pH
Minimal media pH6
+0.4
-0.1
-0.4
+0.1
-1.6
stress
acetate 40 mM
-0.4
+0.1
-0.2
+0.1
-1.1
carbon source
Carbon source Sucrose 2.5 mM
+0.1
+0.1
-0.2
-0.2
-1.4
carbon source
L-Arabinose (C)
+0.2
-0.0
+0.1
-0.0
-1.6
carbon source
Carbon source Sucrose 5 mM
+0.1
+0.2
-0.2
-0.1
-1.3
nitrogen source
L-tyrosine (N)
+0.4
+0.1
-0.0
-0.1
-1.3
nitrogen source
L-Leucine (N)
+0.7
+0.1
-0.3
+0.2
-1.3
carbon source
Carbon source Sucrose 5 mM
+0.2
+0.1
+0.4
-0.2
-1.1
pH
Minimal media pH6
+0.4
+0.2
+0.3
-0.0
-1.1
motility
outer cut, LB soft agar motility assay
+0.7
+0.2
+0.8
-1.2
-0.5
carbon source
D-Xylose (C)
+0.4
+0.1
+0.1
+0.4
-0.9
nitrogen source
Gly-Glu (N)
+0.5
+0.1
+0.2
+0.2
-0.9
carbon source
L-Lysine (C)
+0.2
+0.3
+0.5
+0.2
-0.9
stress
Benzalkonium Chloride 0.016 mg/ml
+0.7
+0.4
+0.6
-0.4
-0.8
carbon source
Ethanol (C)
+0.7
+0.2
+0.5
+0.2
-0.5
stress
perchlorate 75 mM
+0.4
+0.4
+1.1
-0.0
-0.3
nitrogen source
D-Serine (N)
+0.8
+0.2
+0.5
+0.6
-0.3
carbon source
L-Leucine (C)
+0.7
+0.3
+0.5
+0.5
+0.5
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