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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_20410 and AO356_20415 are separated by 134 nucleotides
AO356_20415 and AO356_20420 are separated by 309 nucleotides
AO356_20420 and AO356_20425 are separated by 308 nucleotides
AO356_20425 and AO356_20430 are separated by 78 nucleotides
AO356_20410: AO356_20410 - magnesium transporter, at 4,558,605 to 4,559,576
_20410
AO356_20415: AO356_20415 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 4,559,711 to 4,560,433
_20415
AO356_20420: AO356_20420 - enoyl-CoA hydratase, at 4,560,743 to 4,561,432
_20420
AO356_20425: AO356_20425 - tRNA-Ser, at 4,561,741 to 4,561,830
_20425
AO356_20430: AO356_20430 - hypothetical protein, at 4,561,909 to 4,562,268
_20430
Group
Condition
AO356
_20410
AO356
_20415
AO356
_20420
AO356
_20425
AO356
_20430
motility
outer cut, LB soft agar motility assay
-0.1
-0.4
N.D.
N.D.
-0.1
carbon source
Fumarate (C)
-0.2
+0.0
N.D.
N.D.
-0.3
nitrogen source
Ammonium chloride (N)
-0.1
-0.2
N.D.
N.D.
-0.2
carbon source
D-Fructose (C)
+0.0
-0.3
N.D.
N.D.
-0.2
stress
Cobalt chloride 0.08 mM
-0.1
-0.3
N.D.
N.D.
-0.1
carbon source
Carbon source D-Mannitol 2.5 mM
+0.0
-0.3
N.D.
N.D.
-0.1
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.2
-0.3
N.D.
N.D.
+0.1
carbon source
succinate (C)
+0.1
+0.0
N.D.
N.D.
-0.4
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.1
-0.3
N.D.
N.D.
+0.1
carbon source
Carbon source D-Mannitol 5 mM
+0.2
-0.3
N.D.
N.D.
-0.0
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.1
-0.3
N.D.
N.D.
+0.3
carbon source
D-Trehalose (C)
+0.1
-0.3
N.D.
N.D.
+0.1
motility
outer cut, LB soft agar motility assay
-0.0
-0.3
N.D.
N.D.
+0.3
stress
Chlorite 0.04 mM
+0.1
+0.1
N.D.
N.D.
-0.2
nitrogen source
Urea (N)
-0.3
+0.1
N.D.
N.D.
+0.3
carbon source
Carbon source D-Mannitol 2.5 mM
+0.1
-0.2
N.D.
N.D.
+0.1
stress
Benzalkonium Chloride 0.008 mg/ml
+0.1
+0.2
N.D.
N.D.
-0.2
stress
Lomefloxacin 0.0005 mM
-0.1
-0.0
N.D.
N.D.
+0.4
stress
Sisomicin 0.001 mg/ml
+0.0
-0.3
N.D.
N.D.
+0.4
anaerobic
Nitrate reduction with yeast extract
+0.1
+0.3
N.D.
N.D.
-0.1
nitrogen source
L-Isoleucine (N)
-0.1
+0.2
N.D.
N.D.
+0.2
stress
Choline chloride 500 mM
+0.2
+0.2
N.D.
N.D.
-0.1
stress
Rifampicin 0.0024 mg/ml
-0.0
+0.3
N.D.
N.D.
+0.2
lb
LB
+0.1
+0.3
N.D.
N.D.
+0.1
stress
Nalidixic 0.004 mg/ml
+0.1
+0.1
N.D.
N.D.
+0.2
motility
outer cut, LB soft agar motility assay
+0.1
+0.2
N.D.
N.D.
+0.2
stress
Ceftazidime 0.2 mg/ml
+0.1
+0.2
N.D.
N.D.
+0.2
motility
outer cut, LB soft agar motility assay
+0.2
+0.0
N.D.
N.D.
+0.3
stress
Cholin acetate 40 mM
+0.1
+0.3
N.D.
N.D.
+0.3
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.1
+0.6
N.D.
N.D.
+0.2
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