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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_20095 and AO356_20100 are separated by 279 nucleotides
AO356_20100 and AO356_20105 overlap by 4 nucleotides
AO356_20105 and AO356_20110 overlap by 1 nucleotides
AO356_20110 and AO356_20115 are separated by 20 nucleotides
AO356_20095: AO356_20095 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 4,495,464 to 4,496,483
_20095
AO356_20100: AO356_20100 - (2Fe-2S)-binding protein, at 4,496,763 to 4,497,233
_20100
AO356_20105: AO356_20105 - acylaldehyde oxidase, at 4,497,230 to 4,499,545
_20105
AO356_20110: AO356_20110 - hypothetical protein, at 4,499,545 to 4,500,516
_20110
AO356_20115: AO356_20115 - molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 4,500,537 to 4,501,127
_20115
Group
Condition
AO356
_20095
AO356
_20100
AO356
_20105
AO356
_20110
AO356
_20115
motility
outer cut, LB soft agar motility assay
N.D.
-0.5
-0.0
-0.7
-0.1
stress
methylglyoxal 0.016 vol%
N.D.
-0.3
-0.2
+0.0
-0.4
pH
Minimal media pH9
N.D.
-0.4
-0.0
-0.4
-0.2
stress
Thallium(I) acetate 0.008 mg/ml
N.D.
-0.4
-0.1
-0.0
-0.1
stress
Cholin acetate 40 mM
N.D.
-0.4
-0.3
+0.1
-0.1
carbon source
L-Arginine (C)
N.D.
-0.1
-0.1
-0.1
-0.4
survival
4C survival; 1152 hrs
N.D.
-0.1
-0.2
+0.0
-0.3
anaerobic
Nitrate reduction with yeast extract
N.D.
-0.2
+0.1
-0.2
-0.3
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
N.D.
-0.9
+0.2
+0.3
-0.0
stress
Chlorite 0.04 mM
N.D.
+0.1
-0.1
+0.0
-0.5
temperature
LB 30C
N.D.
-0.3
-0.1
-0.2
+0.1
stress
Choline chloride 500 mM
N.D.
+0.2
-0.2
-0.2
-0.2
phage
P5ss-5 phage 10-1 dilution SM calcl2
N.D.
+0.3
+0.1
-0.5
-0.2
nitrogen source
Adenine (N)
N.D.
+0.3
-0.0
-0.3
-0.2
carbon source
L-Aspartic Acid (C)
N.D.
+0.1
+0.0
+0.1
-0.5
motility
outer cut, LB soft agar motility assay
N.D.
+0.3
-0.1
-0.2
-0.2
motility
outer cut, LB soft agar motility assay
N.D.
+0.1
+0.1
+0.2
-0.6
carbon source
Carbon source Sucrose 10 mM
N.D.
+0.3
+0.1
-0.2
-0.4
stress
copper (II) chloride 2.5 mM
N.D.
-0.2
+0.1
-0.2
+0.2
stress
KB-half, Pseudo5 supernatant
N.D.
-0.5
+0.1
+0.2
+0.1
survival
4C survival; 192 hrs
N.D.
+0.3
-0.2
-0.2
+0.1
carbon source
D-Galactose (C)
N.D.
+0.1
-0.2
-0.2
+0.2
survival
4C survival; 360 hrs
N.D.
+0.2
-0.0
-0.3
+0.1
stress
Benzalkonium Chloride 0.016 mg/ml
N.D.
+0.2
+0.1
+0.1
-0.4
stress
1-ethyl-3-methylimidazolium acetate 5 mM
N.D.
-0.3
+0.1
+0.1
+0.2
carbon source
L-Arabinose (C)
N.D.
-0.2
+0.0
-0.2
+0.5
nitrogen source
L-tyrosine (N)
N.D.
+0.4
-0.1
+0.2
-0.2
nitrogen source
Urea (N)
N.D.
+0.3
+0.1
-0.1
+0.1
stress
Rifampicin 0.0016 mg/ml
N.D.
+0.2
+0.2
-0.1
+0.3
carbon source
succinate (C)
N.D.
+0.2
+0.0
-0.0
+0.3
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