Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_20055 and AO356_20060 are separated by 146 nucleotidesAO356_20060 and AO356_20065 are separated by 65 nucleotidesAO356_20065 and AO356_20070 overlap by 4 nucleotidesAO356_20070 and AO356_20075 overlap by 1 nucleotides AO356_20055: AO356_20055 - ATP-binding protein, at 4,488,959 to 4,489,474 _20055 AO356_20060: AO356_20060 - competence protein ComEC, at 4,489,621 to 4,491,867 _20060 AO356_20065: AO356_20065 - biopolymer transporter ExbB, at 4,491,933 to 4,492,598 _20065 AO356_20070: AO356_20070 - biopolymer transporter ExbD, at 4,492,595 to 4,493,023 _20070 AO356_20075: AO356_20075 - tetraacyldisaccharide 4'-kinase, at 4,493,023 to 4,494,033 _20075
Group Condition AO356_20055 AO356_20060 AO356_20065 AO356_20070 AO356_20075
stress 1-ethyl-3-methylimidazolium lysine 10 mM -2.5 -0.1 N.D. N.D. N.D.
stress Benzalkonium Chloride 0.016 mg/ml -1.3 +0.0 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -0.2 -0.5 N.D. N.D. N.D.
stress KB-half, Pseudo11 supernatant, 100 uM FeCl3 -0.7 -0.0 N.D. N.D. N.D.
pH Minimal media pH9 -0.5 -0.2 N.D. N.D. N.D.
carbon source Fumarate (C) -0.3 -0.1 N.D. N.D. N.D.
carbon source D-Trehalose (C) -0.4 +0.1 N.D. N.D. N.D.
carbon source Fumarate (C) -0.4 +0.0 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay +0.1 -0.3 N.D. N.D. N.D.
stress methylglyoxal 0.016 vol% +0.1 -0.3 N.D. N.D. N.D.
survival 4C survival; 192 hrs -0.5 +0.3 N.D. N.D. N.D.
stress Doxycycline hyclate 0.0001 mg/ml -0.3 +0.1 N.D. N.D. N.D.
carbon source L-Glutamine (C) +0.1 -0.3 N.D. N.D. N.D.
stress KB-half, Pseudo5 supernatant -0.5 +0.4 N.D. N.D. N.D.
stress Cisplatin 0.0032 mg/ml +0.2 -0.3 N.D. N.D. N.D.
survival 4C survival; 1152 hrs -0.3 +0.3 N.D. N.D. N.D.
stress 1-ethyl-3-methylimidazolium acetate 20 mM +0.3 -0.3 N.D. N.D. N.D.
stress Aluminum chloride 5 mM +0.2 -0.2 N.D. N.D. N.D.
phage P5ss-5 phage 10-1 dilution SM calcl2 -0.3 +0.3 N.D. N.D. N.D.
stress Cholin acetate 20 mM +0.2 -0.1 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay +0.3 -0.2 N.D. N.D. N.D.
carbon source L-Histidine (C) -0.2 +0.3 N.D. N.D. N.D.
stress Cisplatin 0.0032 mg/ml +0.3 -0.1 N.D. N.D. N.D.
stress Sisomicin 0.001 mg/ml +0.3 -0.1 N.D. N.D. N.D.
nitrogen source Uridine (N) +0.3 -0.1 N.D. N.D. N.D.
carbon source D-Sorbitol (C) +0.3 +0.1 N.D. N.D. N.D.
temperature LB 10C +0.3 +0.1 N.D. N.D. N.D.
carbon source pyruvate (C) +0.2 +0.1 N.D. N.D. N.D.
stress Nalidixic 0.004 mg/ml +0.2 +0.2 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay +0.6 +0.0 N.D. N.D. N.D.
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