Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_19900 and AO356_19905 are separated by 11 nucleotidesAO356_19905 and AO356_19910 are separated by 60 nucleotidesAO356_19910 and AO356_19915 overlap by 4 nucleotidesAO356_19915 and AO356_19920 are separated by 102 nucleotides AO356_19900: AO356_19900 - lysine transporter LysE, at 4,458,565 to 4,459,197 _19900 AO356_19905: AO356_19905 - aminotransferase, at 4,459,209 to 4,460,465 _19905 AO356_19910: AO356_19910 - cystathionine gamma-synthase, at 4,460,526 to 4,461,671 _19910 AO356_19915: AO356_19915 - pyruvate formate lyase-activating protein, at 4,461,668 to 4,463,152 _19915 AO356_19920: AO356_19920 - hypothetical protein, at 4,463,255 to 4,463,968 _19920
Group Condition AO356_19900 AO356_19905 AO356_19910 AO356_19915 AO356_19920
carbon source Putrescine (C) -3.8 -0.1 +0.1 +0.2 +0.1
carbon source NAG (C) -3.3 -0.0 +0.0 +0.0 -0.2
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine -3.6 -0.1 +0.1 +0.1 +0.2
resistance 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine -3.3 -0.1 -0.0 +0.0 +0.1
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -3.5 -0.0 +0.1 +0.1 +0.1
carbon source L-Proline (C) -2.9 +0.1 +0.1 +0.1 -0.1
carbon source L-Proline (C) -3.1 -0.0 +0.2 +0.1 +0.1
pH Minimal media pH9 -2.4 -0.1 +0.0 -0.1 +0.2
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -3.5 +0.2 +0.3 +0.4 +0.6
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine -1.9 -0.0 -0.0 -0.0 +0.1
resistance 20 mM putrescine -1.7 +0.1 +0.0 -0.0 -0.1
carbon source D-Glucosamine Hydrochloride (C) -1.8 +0.1 +0.0 +0.0 +0.3
resistance 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine -1.5 -0.0 +0.0 +0.1 +0.1
carbon source D-Fructose (C) -1.1 +0.0 +0.1 -0.1 -0.1
carbon source L-Lysine (C) -0.4 -0.1 -0.2 -0.2 -0.1
carbon source L-Asparagine (C) -0.5 -0.0 -0.1 -0.0 -0.3
carbon source L-Aspartic Acid (C) -1.2 +0.1 +0.1 +0.2 -0.0
carbon source L-Arginine (C) -1.5 +0.2 +0.1 +0.1 +0.2
carbon source D-Galactose (C) -0.7 +0.0 -0.0 -0.0 -0.0
carbon source L-Arginine (C) -1.1 +0.0 -0.0 +0.1 +0.1
anaerobic Nitrate reduction with yeast extract -0.1 +0.2 +0.0 -0.5 -0.2
carbon source L-Glutamine (C) -0.8 +0.0 +0.1 +0.1 -0.1
carbon source L-Glutamine (C) -0.6 +0.0 +0.1 -0.1 -0.1
stress Cholin acetate 40 mM +0.1 -0.3 -0.2 +0.1 -0.3
carbon source L-Aspartic Acid (C) -0.6 +0.1 +0.1 -0.1 +0.1
carbon source D-Galactose (C) -0.6 +0.2 +0.1 +0.1 +0.0
stress Cephalothin 2 mg/ml +0.1 +0.0 -0.0 +0.2 -0.5
motility outer cut, LB soft agar motility assay -0.3 +0.1 +0.2 -0.2 +0.2
carbon source pyruvate (C) -0.1 +0.2 +0.1 +0.2 +0.3
motility outer cut, LB soft agar motility assay +0.3 +0.0 +0.3 +0.3 +0.4
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