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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_19850 and AO356_19855 are separated by 120 nucleotides
AO356_19855 and AO356_19860 are separated by 224 nucleotides
AO356_19860 and AO356_19865 are separated by 206 nucleotides
AO356_19865 and AO356_19870 are separated by 110 nucleotides
AO356_19850: AO356_19850 - orotidine 5'-phosphate decarboxylase, at 4,450,092 to 4,450,790
_19850
AO356_19855: AO356_19855 - NADP-dependent oxidoreductase, at 4,450,911 to 4,451,915
_19855
AO356_19860: AO356_19860 - short-chain dehydrogenase, at 4,452,140 to 4,452,901
_19860
AO356_19865: AO356_19865 - GntR family transcriptional regulator, at 4,453,108 to 4,454,496
_19865
AO356_19870: AO356_19870 - chemotaxis protein, at 4,454,607 to 4,456,574
_19870
Group
Condition
AO356
_19850
AO356
_19855
AO356
_19860
AO356
_19865
AO356
_19870
motility
outer cut, LB soft agar motility assay
N.D.
-0.1
-0.1
-0.7
-0.1
nitrogen source
Urea (N)
N.D.
-0.2
-0.2
-0.2
-0.2
stress
Choline chloride 300 mM
N.D.
-0.2
-0.1
-0.3
-0.1
carbon source
L-Lysine (C)
N.D.
-0.3
-0.3
+0.0
-0.2
stress
1-ethyl-3-methylimidazolium chloride 200 mM
N.D.
-0.4
-0.3
+0.1
+0.0
carbon source
L-Glutamine (C)
N.D.
-0.0
-0.0
-0.4
-0.1
motility
outer cut, LB soft agar motility assay
N.D.
+0.0
-0.0
-0.4
-0.2
carbon source
L-Histidine (C)
N.D.
-0.2
-0.2
-0.3
+0.2
lb
LB
N.D.
+0.1
-0.2
-0.4
+0.1
nitrogen source
Adenine (N)
N.D.
+0.1
-0.1
-0.1
-0.3
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
N.D.
+0.2
+0.2
-0.3
-0.5
carbon source
D-Alanine (C)
N.D.
-0.2
-0.2
-0.1
+0.2
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
N.D.
-0.0
-0.1
-0.3
+0.2
stress
Sisomicin 0.001 mg/ml
N.D.
-0.1
-0.2
+0.3
-0.3
stress
sodium fluoride 6.25 mM
N.D.
+0.2
-0.0
-0.4
-0.1
carbon source
L-Glutamic (C)
N.D.
-0.1
+0.1
-0.2
+0.1
pH
Minimal media pH6
N.D.
-0.1
-0.1
+0.3
-0.1
survival
4C survival; 1152 hrs
N.D.
+0.0
-0.1
+0.3
-0.3
stress
Spectinomycin 0.025 mg/ml
N.D.
-0.2
+0.2
-0.3
+0.2
stress
1-ethyl-3-methylimidazolium lysine 10 mM
N.D.
-0.1
-0.2
+0.2
+0.1
nitrogen source
D-Serine (N)
N.D.
+0.2
-0.3
+0.1
+0.1
carbon source
Carbon source D,L-Lactate 10 mM
N.D.
-0.3
+0.0
+0.3
-0.1
stress
Nickel (II) chloride 0.625 mM
N.D.
-0.2
+0.1
+0.1
+0.2
carbon source
Carbon source Sucrose 2.5 mM
N.D.
+0.1
-0.1
+0.3
-0.1
phage
P5ss-5 phage 10-1 dilution SM calcl2
N.D.
+0.3
+0.0
-0.2
+0.1
stress
KB-half, Pseudo5 supernatant
N.D.
+0.0
+0.4
+0.1
-0.3
stress
Chlorite 0.04 mM
N.D.
-0.0
+0.1
+0.4
-0.1
anaerobic
Nitrate reduction with yeast extract
N.D.
+0.0
-0.3
+0.7
+0.0
carbon source
Carbon source D-Mannitol 10 mM
N.D.
-0.0
+0.1
+0.4
+0.1
carbon source
Carbon source Sucrose 10 mM
N.D.
+0.3
+0.1
+0.3
-0.0
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