Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_19280 and AO356_19285 are separated by 17 nucleotidesAO356_19285 and AO356_19290 are separated by 92 nucleotidesAO356_19290 and AO356_19295 are separated by 31 nucleotidesAO356_19295 and AO356_19300 are separated by 269 nucleotides AO356_19280: AO356_19280 - tRNA-Val, at 4,327,065 to 4,327,140 _19280 AO356_19285: AO356_19285 - tRNA-Asp, at 4,327,158 to 4,327,234 _19285 AO356_19290: AO356_19290 - catalase, at 4,327,327 to 4,328,463 _19290 AO356_19295: AO356_19295 - hypothetical protein, at 4,328,495 to 4,330,300 _19295 AO356_19300: AO356_19300 - peptidylprolyl isomerase, at 4,330,570 to 4,331,283 _19300
Group Condition AO356_19280 AO356_19285 AO356_19290 AO356_19295 AO356_19300
motility outer cut, LB soft agar motility assay N.D. -1.2 +0.2 +0.1 -2.3
motility outer cut, LB soft agar motility assay N.D. -1.1 +0.0 +0.2 -2.0
motility outer cut, LB soft agar motility assay N.D. +0.1 +0.1 +0.1 -2.9
stress Nickel (II) chloride 0.625 mM N.D. -2.4 +0.0 +0.1 -0.4
carbon source Carbon source Sucrose 2.5 mM N.D. -2.7 +0.1 -0.2 +0.3
motility outer cut, LB soft agar motility assay N.D. -0.0 +0.1 -0.0 -2.2
survival 4C survival; 360 hrs N.D. -1.0 -0.1 +0.0 -0.9
stress Choline chloride 500 mM N.D. -0.4 -0.1 +0.1 -1.5
carbon source Carbon source D-Mannitol 2.5 mM N.D. -1.8 -0.1 -0.0 +0.1
stress nitrite 10 mM N.D. -1.1 -0.0 -0.1 -0.4
stress Bacitracin 2 mg/ml N.D. -1.3 -0.0 +0.0 -0.3
survival 4C survival; 672 hrs N.D. -0.8 +0.1 +0.0 -1.0
nitrogen source L-Isoleucine (N) N.D. -1.9 -0.1 -0.0 +0.4
stress Chlorite 0.04 mM N.D. -1.3 +0.1 -0.1 -0.1
carbon source Carbon source Sucrose 10 mM N.D. -1.9 +0.1 -0.1 +0.5
survival 4C survival; 1152 hrs N.D. -0.7 +0.2 +0.0 -0.8
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. +0.4 -0.1 -0.1 -1.5
stress methylglyoxal 0.016 vol% N.D. -0.8 +0.3 +0.1 -0.6
carbon source D-Trehalose (C) N.D. -1.3 +0.0 -0.0 +0.4
carbon source Carbon source Sucrose 10 mM N.D. -1.2 +0.1 -0.0 +0.6
carbon source L-Isoleucine (C) N.D. -1.2 -0.1 +0.0 +0.7
survival 4C survival; 192 hrs N.D. +0.6 +0.1 +0.1 -1.2
carbon source L-Lysine (C) N.D. +0.7 +0.1 +0.1 -0.9
stress 1-ethyl-3-methylimidazolium acetate 20 mM N.D. +0.9 -0.0 -0.0 -0.8
stress copper (II) chloride 2.5 mM N.D. +1.3 -0.2 -0.1 -0.5
carbon source NAG (C) N.D. -0.2 -0.0 -0.2 +1.2
phage P5ss-5 phage 10-1 dilution SM calcl2 N.D. +1.8 -0.3 -0.2 +0.0
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. +0.6 +0.0 -0.2 +1.1
carbon source Glycerol (C) N.D. +1.2 +0.1 +0.0 +0.3
stress Cisplatin 0.0032 mg/ml N.D. +1.1 +0.3 +0.0 +0.7
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