Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_18950 and AO356_18955 are separated by 170 nucleotidesAO356_18955 and AO356_18960 are separated by 144 nucleotidesAO356_18960 and AO356_18965 are separated by 105 nucleotidesAO356_18965 and AO356_18970 overlap by 4 nucleotides AO356_18950: AO356_18950 - 4'-phosphopantetheinyl transferase, at 4,261,436 to 4,262,170 _18950 AO356_18955: AO356_18955 - dienelactone hydrolase, at 4,262,341 to 4,263,132 _18955 AO356_18960: AO356_18960 - hypothetical protein, at 4,263,277 to 4,263,876 _18960 AO356_18965: AO356_18965 - transcriptional regulator, at 4,263,982 to 4,264,659 _18965 AO356_18970: AO356_18970 - histidine kinase, at 4,264,656 to 4,266,002 _18970
Group Condition AO356_18950 AO356_18955 AO356_18960 AO356_18965 AO356_18970
stress 1-ethyl-3-methylimidazolium lysine 10 mM N.D. +0.2 -3.6 -3.6 -2.8
stress Bacitracin 2 mg/ml N.D. -0.0 -2.5 -2.0 -1.0
stress Cephalothin 2 mg/ml N.D. -0.1 -1.9 -1.6 -1.3
stress Cholin acetate 40 mM N.D. +0.0 -1.5 -1.7 -1.6
survival 4C survival; 1152 hrs N.D. +0.2 -1.1 -1.7 -1.4
stress acetate 40 mM N.D. +0.1 -1.3 -1.7 -0.9
carbon source L-Lysine (C) N.D. -0.4 -0.3 -0.9 -2.2
phage P5ss-5 phage 10-1 dilution SM calcl2 N.D. -0.0 -0.7 -1.4 -1.2
carbon source L-Lysine (C) N.D. +0.0 -0.4 -0.4 -2.3
stress Benzalkonium Chloride 0.016 mg/ml N.D. -0.2 -2.4 -0.0 -0.2
pH Minimal media pH9 N.D. -0.0 -0.3 -1.1 -1.1
survival 4C survival; 672 hrs N.D. +0.0 -0.8 -0.7 -1.0
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. -0.3 -0.5 -0.6 -1.1
nitrogen source D-Glucosamine Hydrochloride (N) N.D. -0.0 -0.0 -0.4 -1.9
stress Lomefloxacin 0.0005 mM N.D. -0.4 -1.3 -0.5 +0.1
stress Cholin acetate 20 mM N.D. +0.1 -1.0 -0.6 -0.6
stress 1-ethyl-3-methylimidazolium acetate 20 mM N.D. +0.3 -0.5 -1.0 -0.7
stress Benzethonium chloride 0.025 mM N.D. +0.0 -1.6 -0.1 -0.1
survival 4C survival; 360 hrs N.D. +0.2 -0.4 -0.8 -0.7
stress 1-ethyl-3-methylimidazolium acetate 10 mM N.D. +0.1 -0.6 -0.5 -0.6
stress Aluminum chloride 5 mM N.D. +0.1 +0.1 -1.1 -0.6
nitrogen source L-Phenylalanine (N) N.D. -0.1 +0.3 +0.5 -0.8
stress methylglyoxal 0.016 vol% N.D. -0.3 -0.5 +0.5 +0.5
stress Nalidixic 0.004 mg/ml N.D. +0.1 -0.4 +0.7 +1.0
stress Choline chloride 300 mM N.D. +0.4 +0.4 +0.7 +0.6
stress Doxycycline hyclate 0.0001 mg/ml N.D. -0.2 -0.1 +1.4 +1.2
stress Choline chloride 500 mM N.D. +0.4 +2.4 -0.1 -0.0
stress Spectinomycin 0.025 mg/ml N.D. +0.1 +0.0 +1.4 +1.3
stress Rifampicin 0.0024 mg/ml N.D. +0.3 +0.2 +1.6 +1.4
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. +0.2 +1.8 +1.2 +1.3
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