Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_18645 and AO356_18650 are separated by 82 nucleotidesAO356_18650 and AO356_18655 are separated by 371 nucleotidesAO356_18655 and AO356_18660 overlap by 4 nucleotidesAO356_18660 and AO356_18665 are separated by 91 nucleotides AO356_18645: AO356_18645 - quinone oxidoreductase, at 4,189,911 to 4,190,825 _18645 AO356_18650: AO356_18650 - lysine transporter LysE, at 4,190,908 to 4,191,528 _18650 AO356_18655: AO356_18655 - toxin HipA, at 4,191,900 to 4,193,216 _18655 AO356_18660: AO356_18660 - transcriptional regulator, at 4,193,213 to 4,193,617 _18660 AO356_18665: AO356_18665 - antitoxin, at 4,193,709 to 4,193,954 _18665
Group Condition AO356_18645 AO356_18650 AO356_18655 AO356_18660 AO356_18665
survival 4C survival; 1152 hrs -0.1 -0.6 -0.0 N.D. -0.1
phage P5ss-5 phage 10-1 dilution SM calcl2 -0.1 -0.2 -0.1 N.D. -0.3
temperature LB 20C -0.2 -0.1 -0.1 N.D. -0.2
stress Lomefloxacin 0.0005 mM -0.2 +0.1 -0.0 N.D. -0.5
pH Minimal media pH9 +0.1 -0.2 -0.0 N.D. -0.3
survival 4C survival; 672 hrs -0.1 -0.5 +0.1 N.D. +0.1
stress 1-ethyl-3-methylimidazolium lysine 10 mM -0.3 -0.0 -0.1 N.D. +0.2
stress Ceftazidime 0.2 mg/ml -0.2 +0.2 +0.1 N.D. -0.3
stress Benzalkonium Chloride 0.016 mg/ml +0.1 +0.1 -0.1 N.D. -0.2
resistance 20 mM putrescine -0.2 +0.1 +0.2 N.D. -0.1
carbon source Carbon source Sucrose 10 mM +0.2 +0.1 +0.0 N.D. -0.4
carbon source D-Glucose (C) -0.2 -0.1 +0.1 N.D. +0.2
temperature LB 37C -0.2 -0.1 +0.1 N.D. +0.2
survival 4C survival; 360 hrs +0.2 -0.3 +0.0 N.D. +0.0
temperature LB 10C -0.0 -0.2 +0.0 N.D. +0.3
carbon source Ethanol (C) +0.1 -0.0 -0.2 N.D. +0.2
motility outer cut, LB soft agar motility assay -0.4 +0.1 -0.1 N.D. +0.5
carbon source Carbon source D-Mannitol 5 mM +0.1 +0.2 +0.0 N.D. -0.2
carbon source Carbon source D,L-Lactate 10 mM +0.2 +0.1 +0.1 N.D. -0.2
carbon source Carbon source Sucrose 5 mM +0.3 +0.1 +0.1 N.D. -0.2
motility outer cut, LB soft agar motility assay +0.1 -0.0 -0.1 N.D. +0.5
carbon source Carbon source D-Mannitol 10 mM +0.1 +0.2 +0.2 N.D. -0.1
anaerobic Nitrate reduction with yeast extract +0.3 -0.0 -0.0 N.D. +0.2
carbon source Tween 20 (C) +0.1 +0.2 -0.1 N.D. +0.3
carbon source Carbon source Sucrose 10 mM +0.2 +0.0 +0.2 N.D. +0.2
carbon source L-Lactate (C) +0.1 +0.1 +0.2 N.D. +0.3
carbon source Carbon source D-Glucose 10 mM -0.1 +0.2 +0.2 N.D. +0.2
carbon source Glycerol (C) +0.2 +0.1 +0.0 N.D. +0.2
motility outer cut, LB soft agar motility assay +0.1 +0.2 -0.0 N.D. +0.6
motility outer cut, LB soft agar motility assay +0.2 +0.2 -0.1 N.D. +0.6
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