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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_18465 and AO356_18470 are separated by 67 nucleotides
AO356_18470 and AO356_18475 are separated by 226 nucleotides
AO356_18475 and AO356_18480 are separated by 7 nucleotides
AO356_18480 and AO356_18485 are separated by 107 nucleotides
AO356_18465: AO356_18465 - histidine kinase, at 4,157,505 to 4,158,845
_18465
AO356_18470: AO356_18470 - LTXXQ domain protein, at 4,158,913 to 4,159,350
_18470
AO356_18475: AO356_18475 - chemotaxis protein CheY, at 4,159,577 to 4,160,254
_18475
AO356_18480: AO356_18480 - translation initiation factor 2, at 4,160,262 to 4,160,672
_18480
AO356_18485: AO356_18485 - hypothetical protein, at 4,160,780 to 4,161,079
_18485
Group
Condition
AO356
_18465
AO356
_18470
AO356
_18475
AO356
_18480
AO356
_18485
stress
perchlorate 75 mM
-0.3
-0.8
-1.3
+0.1
N.D.
carbon source
Putrescine (C)
-0.1
+0.2
-0.1
-2.1
N.D.
stress
Choline chloride 500 mM
-0.2
-0.5
-0.6
-0.6
N.D.
carbon source
L-Proline (C)
-0.1
-0.2
-0.6
-0.7
N.D.
carbon source
L-Glutamine (C)
-0.1
-0.0
-0.8
-0.7
N.D.
carbon source
NAG (C)
-0.1
+0.0
+0.0
-1.5
N.D.
motility
outer cut, LB soft agar motility assay
-0.0
+0.4
-0.8
-0.9
N.D.
motility
outer cut, LB soft agar motility assay
+0.3
-0.1
-0.5
-1.0
N.D.
stress
Doxycycline hyclate 0.0001 mg/ml
+0.0
+0.2
-0.9
-0.5
N.D.
carbon source
Carbon source D-Mannitol 2.5 mM
-0.0
+0.2
+0.1
-1.5
N.D.
carbon source
L-Lysine (C)
+0.2
-0.6
+0.1
-0.9
N.D.
carbon source
Ethanol (C)
-0.2
+0.2
+0.3
-1.2
N.D.
carbon source
Carbon source D-Mannitol 10 mM
+0.0
+0.4
-0.1
-1.1
N.D.
nitrogen source
Cytosine (N)
+0.0
+0.4
+0.2
-1.2
N.D.
carbon source
Fumarate (C)
-0.0
-0.0
+0.5
-1.0
N.D.
motility
outer cut, LB soft agar motility assay
+0.1
+0.6
-1.1
-0.0
N.D.
carbon source
Carbon source D-Mannitol 2.5 mM
-0.1
-0.1
-0.8
+0.6
N.D.
carbon source
acetate (C)
-0.1
-0.1
+0.9
-1.1
N.D.
nitrogen source
Urea (N)
+0.1
+0.6
+0.5
-1.3
N.D.
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.3
-0.3
-0.5
+0.5
N.D.
pH
Minimal media pH8
+0.0
+0.2
-0.5
+0.8
N.D.
stress
Sisomicin 0.001 mg/ml
+0.3
-1.0
+0.0
+1.4
N.D.
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.2
-0.4
-0.1
+1.1
N.D.
motility
outer cut, LB soft agar motility assay
+0.0
+0.4
+0.3
+0.8
N.D.
carbon source
D-Galactose (C)
+0.2
+0.3
+0.6
+0.5
N.D.
anaerobic
Nitrate reduction with yeast extract
-0.2
+0.3
+1.2
+0.5
N.D.
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.2
+0.5
+0.2
+1.5
N.D.
carbon source
L-Arabinose (C)
+0.0
+0.4
+0.9
+0.8
N.D.
nitrogen source
Inosine (N)
+0.3
+0.8
+0.9
+1.0
N.D.
stress
KB-half, Pseudo5 supernatant
-0.0
+0.6
-0.1
+3.1
N.D.
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