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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_18165 and AO356_18170 are separated by 238 nucleotides
AO356_18170 and AO356_18175 are separated by 10 nucleotides
AO356_18175 and AO356_18180 are separated by 467 nucleotides
AO356_18180 and AO356_18185 are separated by 219 nucleotides
AO356_18165: AO356_18165 - serine dehydratase, at 4,092,480 to 4,093,856
_18165
AO356_18170: AO356_18170 - glycine dehydrogenase, at 4,094,095 to 4,096,944
_18170
AO356_18175: AO356_18175 - glycine cleavage system protein H, at 4,096,955 to 4,097,338
_18175
AO356_18180: AO356_18180 - Fis family transcriptional regulator, at 4,097,806 to 4,099,314
_18180
AO356_18185: AO356_18185 - LysR family transcriptional regulator, at 4,099,534 to 4,100,439
_18185
Group
Condition
AO356
_18165
AO356
_18170
AO356
_18175
AO356
_18180
AO356
_18185
nitrogen source
Gly-Glu (N)
-1.3
-4.0
-1.3
-1.8
-0.1
stress
copper (II) chloride 2.5 mM
-0.7
-0.7
-0.1
-0.5
+0.1
pH
Minimal media pH9
+0.2
-1.3
-0.3
-0.5
-0.0
nitrogen source
D-Serine (N)
-0.7
-0.5
+0.5
-0.1
-0.1
nitrogen source
L-Phenylalanine (N)
-0.1
-0.6
-0.0
+0.1
-0.0
stress
Cholin acetate 40 mM
-0.2
-0.1
+0.1
+0.2
-0.3
survival
4C survival; 1152 hrs
+0.2
-0.3
+0.0
-0.2
+0.1
pH
Minimal media pH6
+0.2
-0.5
+0.1
-0.0
+0.1
motility
outer cut, LB soft agar motility assay
-0.3
-0.3
+0.3
+0.3
+0.0
anaerobic
Nitrate reduction with yeast extract
+0.2
-0.4
+0.0
+0.0
+0.2
carbon source
acetate (C)
+0.1
-0.4
+0.0
+0.4
-0.1
stress
Benzalkonium Chloride 0.016 mg/ml
+0.2
-0.0
+0.2
+0.1
-0.3
motility
outer cut, LB soft agar motility assay
+0.0
+0.3
+0.3
-0.1
-0.2
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.2
+0.3
-0.3
-0.0
+0.2
stress
Nickel (II) chloride 0.625 mM
+0.3
+0.2
-0.1
+0.4
-0.2
stress
Choline chloride 500 mM
+0.2
+0.2
-0.1
+0.2
+0.1
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.0
-0.1
+0.4
+0.0
+0.3
carbon source
L-Histidine (C)
+0.7
-0.3
-0.1
+0.3
+0.1
stress
Cisplatin 0.0032 mg/ml
+0.0
+0.4
+0.1
+0.3
-0.1
carbon source
L-Lysine (C)
+0.5
+0.1
+0.1
-0.0
+0.2
carbon source
L-Alanine (C)
+0.3
+0.3
+0.2
+0.1
-0.0
carbon source
D-Xylose (C)
+0.2
+0.6
-0.1
+0.1
+0.1
carbon source
L-Lysine (C)
+0.6
-0.0
+0.3
+0.2
+0.0
motility
outer cut, LB soft agar motility assay
+0.2
+0.4
+0.0
+0.2
+0.4
carbon source
L-Histidine (C)
+0.8
-0.3
+0.2
+0.2
+0.3
carbon source
D-Xylose (C)
+0.4
+0.5
+0.0
+0.4
+0.0
stress
Nickel (II) chloride 0.625 mM
+0.6
+0.5
-0.0
+0.5
-0.0
stress
Cobalt chloride 0.08 mM
+0.8
+0.4
+0.1
+0.6
-0.2
stress
KB-half, Pseudo5 supernatant
+0.3
+0.7
-0.0
+0.5
+0.2
stress
Cobalt chloride 0.08 mM
+0.7
+0.5
+0.2
+0.6
-0.1
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