Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_18065 and AO356_18070 are separated by 1 nucleotidesAO356_18070 and AO356_18075 are separated by 63 nucleotidesAO356_18075 and AO356_18080 are separated by 118 nucleotidesAO356_18080 and AO356_18085 are separated by 172 nucleotides AO356_18065: AO356_18065 - 7-carboxy-7-deazaguanine synthase, at 4,072,507 to 4,073,154 _18065 AO356_18070: AO356_18070 - 7-cyano-7-deazaguanine synthase, at 4,073,156 to 4,073,848 _18070 AO356_18075: AO356_18075 - tRNA-Lys, at 4,073,912 to 4,073,987 _18075 AO356_18080: AO356_18080 - LysR family transcriptional regulator, at 4,074,106 to 4,075,041 _18080 AO356_18085: AO356_18085 - thermostable hemolysin, at 4,075,214 to 4,075,831 _18085
Group Condition AO356_18065 AO356_18070 AO356_18075 AO356_18080 AO356_18085
stress Chlorite 0.08 mM -4.7 -2.7 N.D. +0.0 -0.2
stress Chlorite 0.04 mM -3.2 -2.2 N.D. -0.1 +0.0
survival 4C survival; 360 hrs -2.7 -0.2 N.D. -0.2 +0.1
stress Sisomicin 0.001 mg/ml -1.1 -2.1 N.D. +0.3 +0.4
stress Rifampicin 0.0024 mg/ml -1.4 -1.0 N.D. +0.2 -0.1
motility outer cut, LB soft agar motility assay -0.6 -0.8 N.D. -0.8 +0.3
pH Minimal media pH6 -1.1 -0.8 N.D. -0.0 +0.1
carbon source D-Fructose (C) -0.6 -0.7 N.D. -0.3 -0.3
carbon source NAG (C) -0.7 -0.5 N.D. -0.4 +0.1
carbon source D-Glucose (C) -1.2 -0.2 N.D. -0.1 -0.1
stress methylglyoxal 0.016 vol% +0.0 +0.1 N.D. -0.6 -1.0
carbon source Carbon source Sucrose 5 mM -0.1 -0.6 N.D. -0.9 +0.2
stress Aluminum chloride 5 mM -0.7 -0.7 N.D. +0.1 -0.1
carbon source Carbon source Sucrose 5 mM +0.4 -0.7 N.D. -1.1 +0.1
motility outer cut, LB soft agar motility assay -0.5 -1.1 N.D. -0.0 +0.3
carbon source Carbon source D,L-Lactate 10 mM -0.6 -0.5 N.D. -0.3 +0.2
carbon source Carbon source Sucrose 10 mM +0.2 -0.7 N.D. -0.8 +0.1
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -0.5 -0.9 N.D. +0.3 +0.0
carbon source L-Aspartic Acid (C) -1.0 -0.3 N.D. +0.1 +0.3
carbon source L-Malic (C) -1.1 -0.4 N.D. +0.2 +0.5
carbon source L-Malic (C) -0.7 -0.7 N.D. +0.2 +0.4
stress perchlorate 75 mM +0.7 +0.3 N.D. -0.9 -0.8
carbon source L-Glutamine (C) -0.2 -0.5 N.D. +0.5 +0.4
carbon source L-Glutamine (C) -0.5 -0.2 N.D. +0.5 +0.7
stress Choline chloride 500 mM +0.4 +0.7 N.D. -0.3 -0.3
phage P5ss-5 phage 10-1 dilution SM calcl2 +0.8 -0.4 N.D. +0.1 +0.2
stress 1-ethyl-3-methylimidazolium chloride 200 mM -0.0 +0.9 N.D. +0.5 -0.2
nitrogen source D-Serine (N) +0.8 +0.9 N.D. -0.1 -0.2
stress Doxycycline hyclate 0.0001 mg/ml +0.9 +0.8 N.D. +0.1 -0.2
stress Lomefloxacin 0.0005 mM +0.8 +1.2 N.D. +0.3 -0.3
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