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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_17965 and AO356_17970 are separated by 171 nucleotides
AO356_17970 and AO356_17975 are separated by 885 nucleotides
AO356_17975 and AO356_17980 are separated by 3 nucleotides
AO356_17980 and AO356_17985 are separated by 77 nucleotides
AO356_17965: AO356_17965 - mechanosensitive ion channel protein MscS, at 4,055,286 to 4,056,128
_17965
AO356_17970: AO356_17970 - porin, at 4,056,300 to 4,057,553
_17970
AO356_17975: AO356_17975 - histidine triad (HIT) protein, at 4,058,439 to 4,058,864
_17975
AO356_17980: AO356_17980 - hypothetical protein, at 4,058,868 to 4,059,074
_17980
AO356_17985: AO356_17985 - cold-shock protein, at 4,059,152 to 4,059,757
_17985
Group
Condition
AO356
_17965
AO356
_17970
AO356
_17975
AO356
_17980
AO356
_17985
nitrogen source
D-Glucosamine Hydrochloride (N)
+0.4
-4.3
-0.4
N.D.
-1.2
carbon source
D-Glucosamine Hydrochloride (C)
-0.4
-2.2
-0.1
N.D.
-1.7
stress
Nalidixic 0.004 mg/ml
-0.4
+0.0
-0.3
N.D.
-3.4
stress
Lomefloxacin 0.0005 mM
-0.4
-0.5
-0.4
N.D.
-2.6
carbon source
D-Xylose (C)
-0.2
-1.4
-0.3
N.D.
-1.8
carbon source
L-Lysine (C)
-0.5
-2.0
-0.4
N.D.
-0.7
nitrogen source
Inosine (N)
-0.2
-1.6
+0.0
N.D.
-1.7
stress
Bacitracin 2 mg/ml
-0.4
-0.0
+0.0
N.D.
-3.0
stress
Benzalkonium Chloride 0.008 mg/ml
-0.2
+0.2
-0.1
N.D.
-3.1
nitrogen source
Urea (N)
+0.1
-1.6
-0.3
N.D.
-1.2
pH
Minimal media pH6
-0.4
-0.9
-0.3
N.D.
-1.3
carbon source
acetate (C)
-0.1
-0.7
-0.3
N.D.
-1.9
stress
Rifampicin 0.0024 mg/ml
-0.1
+0.1
+0.1
N.D.
-3.0
carbon source
Tween 20 (C)
-0.1
-0.2
-0.1
N.D.
-2.4
stress
methylglyoxal 0.016 vol%
-0.3
+0.4
+0.1
N.D.
-3.0
nitrogen source
D-Serine (N)
-0.3
-2.3
+0.3
N.D.
-0.5
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.3
-1.6
-0.3
N.D.
-0.6
stress
Tetracycline 0.0001 mg/ml
-0.4
-0.1
+0.1
N.D.
-2.4
carbon source
D-Xylose (C)
-0.3
-1.4
-0.5
N.D.
-0.6
pH
Minimal media pH6
-0.2
-1.0
-0.2
N.D.
-1.3
stress
Doxycycline hyclate 0.0001 mg/ml
-0.0
-0.2
-0.5
N.D.
-1.9
stress
Chloramphenicol 0.005 mg/ml
-0.4
+0.0
-0.1
N.D.
-2.1
stress
Benzalkonium Chloride 0.016 mg/ml
+0.4
+0.3
-0.2
N.D.
-3.1
stress
Rifampicin 0.0016 mg/ml
-0.1
+0.2
+0.0
N.D.
-2.6
stress
nitrate 200 mM
-0.1
-0.0
+0.1
N.D.
-2.4
nitrogen source
Adenine (N)
+0.3
-0.7
-0.1
N.D.
-1.7
motility
outer cut, LB soft agar motility assay
+0.4
+0.1
-0.1
N.D.
-2.3
stress
Cisplatin 0.0032 mg/ml
+0.3
-0.0
+0.1
N.D.
-2.3
stress
perchlorate 75 mM
+0.2
+0.7
-0.0
N.D.
-2.4
stress
Aluminum chloride 5 mM
+0.2
+1.3
+0.3
N.D.
-1.9
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