Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_17965 and AO356_17970 are separated by 171 nucleotidesAO356_17970 and AO356_17975 are separated by 885 nucleotidesAO356_17975 and AO356_17980 are separated by 3 nucleotidesAO356_17980 and AO356_17985 are separated by 77 nucleotides AO356_17965: AO356_17965 - mechanosensitive ion channel protein MscS, at 4,055,286 to 4,056,128 _17965 AO356_17970: AO356_17970 - porin, at 4,056,300 to 4,057,553 _17970 AO356_17975: AO356_17975 - histidine triad (HIT) protein, at 4,058,439 to 4,058,864 _17975 AO356_17980: AO356_17980 - hypothetical protein, at 4,058,868 to 4,059,074 _17980 AO356_17985: AO356_17985 - cold-shock protein, at 4,059,152 to 4,059,757 _17985
Group Condition AO356_17965 AO356_17970 AO356_17975 AO356_17980 AO356_17985
nitrogen source D-Glucosamine Hydrochloride (N) +0.4 -4.3 -0.4 N.D. -1.2
carbon source D-Glucosamine Hydrochloride (C) -0.4 -2.2 -0.1 N.D. -1.7
stress Nalidixic 0.004 mg/ml -0.4 +0.0 -0.3 N.D. -3.4
stress Lomefloxacin 0.0005 mM -0.4 -0.5 -0.4 N.D. -2.6
carbon source D-Xylose (C) -0.2 -1.4 -0.3 N.D. -1.8
carbon source L-Lysine (C) -0.5 -2.0 -0.4 N.D. -0.7
nitrogen source Inosine (N) -0.2 -1.6 +0.0 N.D. -1.7
stress Bacitracin 2 mg/ml -0.4 -0.0 +0.0 N.D. -3.0
stress Benzalkonium Chloride 0.008 mg/ml -0.2 +0.2 -0.1 N.D. -3.1
nitrogen source Urea (N) +0.1 -1.6 -0.3 N.D. -1.2
pH Minimal media pH6 -0.4 -0.9 -0.3 N.D. -1.3
carbon source acetate (C) -0.1 -0.7 -0.3 N.D. -1.9
stress Rifampicin 0.0024 mg/ml -0.1 +0.1 +0.1 N.D. -3.0
carbon source Tween 20 (C) -0.1 -0.2 -0.1 N.D. -2.4
stress methylglyoxal 0.016 vol% -0.3 +0.4 +0.1 N.D. -3.0
nitrogen source D-Serine (N) -0.3 -2.3 +0.3 N.D. -0.5
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine -0.3 -1.6 -0.3 N.D. -0.6
stress Tetracycline 0.0001 mg/ml -0.4 -0.1 +0.1 N.D. -2.4
carbon source D-Xylose (C) -0.3 -1.4 -0.5 N.D. -0.6
pH Minimal media pH6 -0.2 -1.0 -0.2 N.D. -1.3
stress Doxycycline hyclate 0.0001 mg/ml -0.0 -0.2 -0.5 N.D. -1.9
stress Chloramphenicol 0.005 mg/ml -0.4 +0.0 -0.1 N.D. -2.1
stress Benzalkonium Chloride 0.016 mg/ml +0.4 +0.3 -0.2 N.D. -3.1
stress Rifampicin 0.0016 mg/ml -0.1 +0.2 +0.0 N.D. -2.6
stress nitrate 200 mM -0.1 -0.0 +0.1 N.D. -2.4
nitrogen source Adenine (N) +0.3 -0.7 -0.1 N.D. -1.7
motility outer cut, LB soft agar motility assay +0.4 +0.1 -0.1 N.D. -2.3
stress Cisplatin 0.0032 mg/ml +0.3 -0.0 +0.1 N.D. -2.3
stress perchlorate 75 mM +0.2 +0.7 -0.0 N.D. -2.4
stress Aluminum chloride 5 mM +0.2 +1.3 +0.3 N.D. -1.9
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