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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_17935 and AO356_17940 overlap by 4 nucleotides
AO356_17940 and AO356_17945 are separated by 101 nucleotides
AO356_17945 and AO356_17950 are separated by 2 nucleotides
AO356_17950 and AO356_17955 are separated by 46 nucleotides
AO356_17935: AO356_17935 - glutathione S-transferase, at 4,049,253 to 4,049,885
_17935
AO356_17940: AO356_17940 - hypothetical protein, at 4,049,882 to 4,050,826
_17940
AO356_17945: AO356_17945 - cobalamin adenosyltransferase, at 4,050,928 to 4,051,509
_17945
AO356_17950: AO356_17950 - histidine kinase, at 4,051,512 to 4,053,548
_17950
AO356_17955: AO356_17955 - 2-dehydropantoate 2-reductase, at 4,053,595 to 4,054,512
_17955
Group
Condition
AO356
_17935
AO356
_17940
AO356
_17945
AO356
_17950
AO356
_17955
anaerobic
Nitrate reduction with yeast extract
-0.7
-0.6
-0.4
-2.5
-1.2
carbon source
L-Isoleucine (C)
+0.0
-1.0
+0.2
-4.5
+0.1
nitrogen source
L-Isoleucine (N)
+0.1
-0.3
-0.1
-3.4
-0.3
carbon source
L-Proline (C)
-0.2
+0.1
-0.2
-3.1
+0.0
carbon source
Carbon source D-Mannitol 5 mM
-0.6
-0.4
-0.5
-1.9
+0.0
carbon source
L-Histidine (C)
+0.0
+0.0
-0.0
-3.6
+0.3
nitrogen source
Ammonium chloride (N)
-0.1
-0.0
+0.0
-3.1
+0.1
carbon source
L-Histidine (C)
-0.1
-0.5
+0.4
-3.2
+0.2
motility
outer cut, LB soft agar motility assay
-0.2
+0.3
-0.4
-2.2
-0.5
carbon source
D-Lactate (C)
+0.1
-0.2
-0.1
-3.1
+0.2
pH
Minimal media pH8
+0.1
-0.5
-0.2
-2.2
-0.4
survival
4C survival; 360 hrs
-0.2
+0.1
-1.1
-1.7
-0.2
pH
Minimal media pH6
-0.0
-0.0
-0.2
-2.6
-0.2
motility
outer cut, LB soft agar motility assay
-0.2
-0.0
-0.6
-1.8
-0.3
carbon source
L-Proline (C)
+0.0
+0.0
-0.0
-2.7
-0.2
pH
Minimal media pH6
-0.2
-0.1
+0.4
-2.4
-0.5
nitrogen source
nitrite (N)
-0.3
-0.0
+0.0
-2.5
-0.1
pH
Minimal media pH7
-0.3
-0.0
-0.0
-2.5
+0.0
motility
outer cut, LB soft agar motility assay
+0.0
+0.1
-0.6
-2.3
+0.1
carbon source
D-Lactate (C)
+0.2
-0.1
-0.3
-3.2
+0.6
carbon source
m-Inositol (C)
+0.1
+0.1
+0.2
-3.2
+0.1
carbon source
m-Inositol (C)
+0.1
+0.0
+0.1
-3.2
+0.4
carbon source
Glycerol (C)
-0.0
-0.0
-0.6
-2.2
+0.2
carbon source
D-Glucose (C)
+0.2
-0.0
-0.7
-1.8
-0.2
carbon source
L-Asparagine (C)
-0.0
+0.2
+0.0
-2.6
+0.0
stress
Sisomicin 0.001 mg/ml
+0.2
+0.2
+0.6
-3.4
+0.2
pH
Minimal media pH7
-0.0
+0.2
+0.1
-2.5
-0.1
carbon source
L-Glutamine (C)
-0.1
-0.2
+0.4
-2.1
-0.1
stress
nitrite 10 mM
+0.4
+0.1
+0.3
-1.7
-0.9
stress
methylglyoxal 0.016 vol%
+0.4
-0.3
+0.2
+2.0
+0.7
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