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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_17315 and AO356_17320 are separated by 810 nucleotides
AO356_17320 and AO356_17325 are separated by 292 nucleotides
AO356_17325 and AO356_17330 are separated by 336 nucleotides
AO356_17330 and AO356_17335 are separated by 256 nucleotides
AO356_17315: AO356_17315 - GDP-mannose dehydrogenase, at 3,902,356 to 3,903,672
_17315
AO356_17320: AO356_17320 - hypothetical protein, at 3,904,483 to 3,905,262
_17320
AO356_17325: AO356_17325 - polysaccharide deacetylase, at 3,905,555 to 3,906,679
_17325
AO356_17330: AO356_17330 - ATPase, at 3,907,016 to 3,908,410
_17330
AO356_17335: AO356_17335 - molybdenum cofactor biosynthesis protein MoaC, at 3,908,667 to 3,909,140
_17335
Group
Condition
AO356
_17315
AO356
_17320
AO356
_17325
AO356
_17330
AO356
_17335
nitrogen source
Inosine (N)
-0.1
+0.1
-0.1
-0.8
-2.4
stress
Chlorite 0.08 mM
+0.1
+0.1
+0.2
-0.1
-3.3
motility
outer cut, LB soft agar motility assay
+0.0
-0.6
-0.8
-0.3
-1.3
carbon source
Carbon source Sucrose 2.5 mM
-0.1
+0.0
+0.1
-1.2
-1.7
carbon source
L-Histidine (C)
+0.2
+0.2
+0.4
+0.3
-3.8
carbon source
Carbon source Sucrose 2.5 mM
+0.0
-0.1
+0.1
-1.4
-1.3
carbon source
L-Histidine (C)
+0.1
+0.1
+0.2
+0.5
-3.5
carbon source
Carbon source Sucrose 5 mM
+0.1
+0.0
+0.1
-1.5
-1.1
carbon source
Carbon source Sucrose 10 mM
-0.0
-0.1
+0.1
-0.8
-1.5
carbon source
Carbon source Sucrose 5 mM
+0.1
-0.0
+0.1
-1.2
-1.2
motility
outer cut, LB soft agar motility assay
+0.1
-0.2
-0.8
+0.2
-1.5
carbon source
Carbon source D-Mannitol 5 mM
-0.0
+0.0
-0.1
-0.4
-1.5
stress
Chlorite 0.04 mM
+0.0
-0.2
+0.3
+0.1
-2.2
carbon source
Carbon source Sucrose 10 mM
+0.1
-0.0
-0.0
-1.0
-0.9
carbon source
Carbon source D-Mannitol 10 mM
-0.0
-0.1
-0.1
-0.8
-0.9
anaerobic
Nitrate reduction with yeast extract
+0.1
+0.2
+0.5
-0.1
-2.5
carbon source
Carbon source D-Mannitol 10 mM
+0.1
+0.1
+0.1
-1.1
-0.9
carbon source
Carnitine Hydrochloride (C)
+0.0
-0.1
+0.0
-0.3
-1.5
carbon source
Carbon source D-Mannitol 2.5 mM
-0.1
+0.2
+0.2
-0.7
-1.1
carbon source
Carbon source D-Mannitol 5 mM
+0.1
-0.1
-0.0
-0.7
-0.9
carbon source
m-Inositol (C)
+0.1
+0.1
+0.1
-1.2
-0.4
motility
outer cut, LB soft agar motility assay
-0.0
-0.2
-0.8
+0.1
-0.4
nitrogen source
Adenine (N)
+0.1
+0.2
+0.1
-0.2
-1.1
stress
Choline chloride 300 mM
+0.3
-0.2
+0.1
-0.0
-1.1
carbon source
L-Lysine (C)
+0.2
+0.2
+0.4
+0.1
-0.9
survival
4C survival; 1152 hrs
+0.9
-0.2
-0.0
-0.0
-0.5
stress
Choline chloride 500 mM
+1.2
+0.3
+0.4
+0.2
-1.1
survival
4C survival; 672 hrs
+1.1
+0.1
+0.0
-0.2
-0.0
stress
syringaldehyde 5 mM
+0.7
+0.4
+0.2
+0.3
-0.2
stress
nitrate 200 mM
+0.6
+0.5
+0.4
+0.2
+0.1
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