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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_17080 and AO356_17085 are separated by 16 nucleotides
AO356_17085 and AO356_17090 are separated by 676 nucleotides
AO356_17090 and AO356_17095 overlap by 4 nucleotides
AO356_17095 and AO356_17100 are separated by 102 nucleotides
AO356_17080: AO356_17080 - stringent starvation protein A, at 3,845,341 to 3,845,958
_17080
AO356_17085: AO356_17085 - peptidase, at 3,845,975 to 3,846,388
_17085
AO356_17090: AO356_17090 - phospholipid-binding protein, at 3,847,065 to 3,847,643
_17090
AO356_17095: AO356_17095 - phosphoheptose isomerase, at 3,847,640 to 3,848,233
_17095
AO356_17100: AO356_17100 - hypothetical protein, at 3,848,336 to 3,848,698
_17100
Group
Condition
AO356
_17080
AO356
_17085
AO356
_17090
AO356
_17095
AO356
_17100
stress
Sisomicin 0.001 mg/ml
-4.7
-0.9
-2.7
N.D.
-0.5
stress
Bacitracin 2 mg/ml
-3.3
+0.1
-1.2
N.D.
+0.2
motility
outer cut, LB soft agar motility assay
-2.2
-0.0
-1.5
N.D.
-0.3
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.3
+0.1
-3.6
N.D.
+0.4
motility
outer cut, LB soft agar motility assay
-1.4
-0.0
-1.0
N.D.
-0.7
stress
acetate 40 mM
-0.8
-0.4
-1.6
N.D.
-0.1
nitrogen source
D-Serine (N)
+0.3
-1.2
-1.5
N.D.
-0.6
motility
outer cut, LB soft agar motility assay
-1.6
+0.3
-1.7
N.D.
-0.0
anaerobic
Nitrate reduction with yeast extract
-1.5
-0.0
-0.9
N.D.
-0.3
carbon source
L-Histidine (C)
-1.8
-0.5
-0.5
N.D.
+0.0
stress
Cholin acetate 40 mM
-0.7
-0.4
-1.8
N.D.
+0.3
stress
Rifampicin 0.0024 mg/ml
-0.8
+0.4
-2.1
N.D.
+0.1
carbon source
L-Histidine (C)
-1.5
-0.2
-0.6
N.D.
-0.1
survival
4C survival; 360 hrs
-0.5
-0.2
-1.3
N.D.
-0.3
stress
1-ethyl-3-methylimidazolium lysine 10 mM
+0.8
-0.1
-3.5
N.D.
+0.6
stress
Cisplatin 0.0032 mg/ml
-0.6
-0.2
-1.7
N.D.
+0.6
carbon source
Ethanol (C)
-1.1
-0.4
-0.5
N.D.
+0.2
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.5
-0.6
-1.9
N.D.
+0.2
carbon source
acetate (C)
-1.3
-0.4
-0.3
N.D.
+0.3
stress
Spectinomycin 0.025 mg/ml
-0.9
+0.3
-1.0
N.D.
+0.1
motility
outer cut, LB soft agar motility assay
-1.5
+0.2
-0.5
N.D.
+0.4
survival
4C survival; 1152 hrs
+1.2
-0.5
-2.0
N.D.
-0.2
stress
Choline chloride 500 mM
+0.6
-0.3
-1.6
N.D.
+0.3
stress
Aluminum chloride 5 mM
-1.4
+0.4
+0.3
N.D.
-0.2
stress
Paraquat dichloride 0.0625 mg/ml
-0.4
+0.4
-1.1
N.D.
+0.6
stress
copper (II) chloride 2.5 mM
-0.0
+0.7
-1.5
N.D.
+0.3
stress
Lomefloxacin 0.0005 mM
-0.9
+0.5
-0.5
N.D.
+0.5
stress
perchlorate 75 mM
+1.2
+0.2
-1.2
N.D.
+0.3
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+1.2
-0.4
-0.3
N.D.
+0.4
stress
Benzalkonium Chloride 0.016 mg/ml
-0.3
+0.5
+1.1
N.D.
+0.6
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