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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_16265 and AO356_16270 are separated by 132 nucleotides
AO356_16270 and AO356_16275 are separated by 10 nucleotides
AO356_16275 and AO356_16280 are separated by 180 nucleotides
AO356_16280 and AO356_16285 are separated by 113 nucleotides
AO356_16265: AO356_16265 - serine kinase/phosphatase, at 3,677,768 to 3,678,172
_16265
AO356_16270: AO356_16270 - carbonate dehydratase, at 3,678,305 to 3,678,949
_16270
AO356_16275: AO356_16275 - lysine methyltransferase, at 3,678,960 to 3,679,487
_16275
AO356_16280: AO356_16280 - hypothetical protein, at 3,679,668 to 3,680,837
_16280
AO356_16285: AO356_16285 - Short chain fatty acid transporter, at 3,680,951 to 3,682,369
_16285
Group
Condition
AO356
_16265
AO356
_16270
AO356
_16275
AO356
_16280
AO356
_16285
nitrogen source
nitrite (N)
N.D.
-4.8
-0.3
+0.0
+0.0
pH
Minimal media pH9
N.D.
-2.4
-0.4
+0.0
-0.1
stress
nitrite 10 mM
N.D.
-2.0
+0.1
+0.1
+0.0
carbon source
Carnitine Hydrochloride (C)
N.D.
-0.8
+0.1
+0.1
-0.2
carbon source
D,L-Lactate (C)
N.D.
-0.6
+0.0
+0.0
-0.0
stress
Benzalkonium Chloride 0.008 mg/ml
N.D.
-0.3
-0.1
-0.1
-0.1
carbon source
D-Lactate (C)
N.D.
-0.5
+0.1
-0.0
-0.1
stress
Vanillin 1.25 mM
N.D.
-0.2
-0.1
-0.2
-0.0
carbon source
L-Leucine (C)
N.D.
-0.3
-0.1
+0.0
-0.1
survival
4C survival; 360 hrs
N.D.
-0.2
-0.1
-0.2
+0.1
nitrogen source
D-Alanine (N)
N.D.
-0.4
-0.1
+0.1
+0.0
pH
Minimal media pH8
N.D.
-0.2
-0.2
+0.2
-0.0
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
N.D.
+0.1
+0.0
-0.4
-0.1
carbon source
Carbon source Sucrose 10 mM
N.D.
-0.3
+0.0
-0.1
+0.1
anaerobic
Nitrate reduction with yeast extract
N.D.
-0.7
+0.2
+0.1
+0.1
carbon source
L-Lactate (C)
N.D.
-0.4
+0.1
+0.1
-0.0
stress
perchlorate 75 mM
N.D.
-0.1
+0.1
-0.2
-0.1
temperature
LB 10C
N.D.
-0.3
+0.1
-0.1
-0.0
stress
Bacitracin 2 mg/ml
N.D.
-0.4
+0.1
+0.1
+0.1
stress
methylglyoxal 0.016 vol%
N.D.
-0.1
+0.2
-0.1
-0.1
pH
Minimal media pH7
N.D.
-0.3
+0.2
+0.1
-0.0
stress
copper (II) chloride 2.5 mM
N.D.
-0.1
-0.2
+0.1
+0.2
lb
LB
N.D.
+0.2
+0.1
-0.1
-0.2
stress
Benzalkonium Chloride 0.016 mg/ml
N.D.
-0.1
+0.1
+0.2
-0.1
motility
outer cut, LB soft agar motility assay
N.D.
+0.1
+0.2
-0.2
+0.0
stress
Chlorite 0.04 mM
N.D.
-0.2
+0.2
-0.1
+0.1
lb
LB
N.D.
-0.2
+0.3
+0.1
+0.0
nitrogen source
D-Serine (N)
N.D.
+0.3
-0.1
+0.2
+0.1
carbon source
L-Histidine (C)
N.D.
+0.2
+0.0
+0.5
+0.0
carbon source
L-Histidine (C)
N.D.
+0.3
+0.1
+0.4
+0.1
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