Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_16255 and AO356_16260 overlap by 8 nucleotidesAO356_16260 and AO356_16265 are separated by 316 nucleotidesAO356_16265 and AO356_16270 are separated by 132 nucleotidesAO356_16270 and AO356_16275 are separated by 10 nucleotides AO356_16255: AO356_16255 - energy transducer TonB, at 3,676,266 to 3,677,006 _16255 AO356_16260: AO356_16260 - ribosomal-protein-alanine acetyltransferase, at 3,676,999 to 3,677,451 _16260 AO356_16265: AO356_16265 - serine kinase/phosphatase, at 3,677,768 to 3,678,172 _16265 AO356_16270: AO356_16270 - carbonate dehydratase, at 3,678,305 to 3,678,949 _16270 AO356_16275: AO356_16275 - lysine methyltransferase, at 3,678,960 to 3,679,487 _16275
Group Condition AO356_16255 AO356_16260 AO356_16265 AO356_16270 AO356_16275
nitrogen source nitrite (N) N.D. -0.3 N.D. -4.8 -0.3
pH Minimal media pH9 N.D. -0.4 N.D. -2.4 -0.4
stress nitrite 10 mM N.D. -0.6 N.D. -2.0 +0.1
stress Cobalt chloride 0.08 mM N.D. -2.7 N.D. +0.1 +0.1
stress Cobalt chloride 0.08 mM N.D. -1.9 N.D. +0.1 -0.0
stress Benzalkonium Chloride 0.016 mg/ml N.D. -1.8 N.D. -0.1 +0.1
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. -1.4 N.D. -0.1 -0.1
stress Nickel (II) chloride 0.625 mM N.D. -1.6 N.D. +0.1 -0.1
anaerobic Nitrate reduction with yeast extract N.D. -1.1 N.D. -0.7 +0.2
phage P5ss-5 phage 10-1 dilution SM calcl2 N.D. -1.5 N.D. -0.0 -0.1
survival 4C survival; 360 hrs N.D. -0.9 N.D. -0.2 -0.1
survival 4C survival; 1152 hrs N.D. -1.2 N.D. +0.2 -0.1
carbon source Inosine (C) N.D. -0.8 N.D. -0.2 -0.1
stress Spectinomycin 0.025 mg/ml N.D. -0.9 N.D. -0.1 +0.1
carbon source Carbon source Sucrose 10 mM N.D. -0.7 N.D. -0.3 +0.0
stress Benzalkonium Chloride 0.008 mg/ml N.D. -0.6 N.D. -0.3 -0.1
stress Nickel (II) chloride 0.625 mM N.D. -0.9 N.D. +0.1 +0.0
carbon source D-Gluconic Acid (C) N.D. -0.9 N.D. +0.1 +0.1
motility outer cut, LB soft agar motility assay N.D. -0.8 N.D. +0.1 +0.2
nitrogen source D-Serine (N) N.D. -0.6 N.D. +0.3 -0.1
stress copper (II) chloride 2.5 mM N.D. +0.9 N.D. -0.1 -0.2
carbon source L-Leucine (C) N.D. +0.9 N.D. -0.3 -0.1
carbon source Ethanol (C) N.D. +1.0 N.D. -0.0 -0.0
carbon source L-Lysine (C) N.D. +1.0 N.D. +0.2 -0.1
carbon source L-Histidine (C) N.D. +0.9 N.D. +0.2 +0.0
carbon source L-Histidine (C) N.D. +0.7 N.D. +0.3 +0.1
carbon source Ethanol (C) N.D. +1.1 N.D. +0.0 +0.1
carbon source L-Lysine (C) N.D. +1.2 N.D. +0.1 -0.1
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. +1.2 N.D. +0.1 +0.1
nitrogen source L-tyrosine (N) N.D. +1.4 N.D. +0.2 +0.1
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