Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_16045 and AO356_16050 are separated by 200 nucleotidesAO356_16050 and AO356_16055 are separated by 327 nucleotidesAO356_16055 and AO356_16060 overlap by 4 nucleotidesAO356_16060 and AO356_16065 are separated by 365 nucleotides AO356_16045: AO356_16045 - acetyl-CoA synthetase, at 3,627,147 to 3,629,084 _16045 AO356_16050: AO356_16050 - glucose-6-phosphate isomerase, at 3,629,285 to 3,630,949 _16050 AO356_16055: AO356_16055 - pantoate--beta-alanine ligase, at 3,631,277 to 3,632,137 _16055 AO356_16060: AO356_16060 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,632,134 to 3,632,934 _16060 AO356_16065: AO356_16065 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 3,633,300 to 3,633,785 _16065
Group Condition AO356_16045 AO356_16050 AO356_16055 AO356_16060 AO356_16065
nitrogen source L-Isoleucine (N) -0.2 -3.3 N.D. -2.4 N.D.
carbon source NAG (C) -0.7 -3.4 N.D. -1.2 N.D.
pH Minimal media pH9 +0.0 -3.3 N.D. -1.9 N.D.
nitrogen source L-Leucine (N) +0.0 -3.4 N.D. -1.7 N.D.
nitrogen source Thymine (N) -0.0 -4.0 N.D. -0.9 N.D.
carbon source L-Histidine (C) -0.0 -2.8 N.D. -2.1 N.D.
motility outer cut, LB soft agar motility assay -0.1 -3.2 N.D. -1.5 N.D.
nitrogen source D-Glucosamine Hydrochloride (N) +0.0 -4.0 N.D. -0.8 N.D.
nitrogen source Inosine (N) -0.0 -3.9 N.D. -0.6 N.D.
nitrogen source L-Phenylalanine (N) -0.0 -3.4 N.D. -1.1 N.D.
carbon source L-Histidine (C) +0.0 -2.9 N.D. -1.6 N.D.
nitrogen source Adenosine (N) -0.1 -4.0 N.D. -0.3 N.D.
nitrogen source Cytosine (N) +0.1 -3.6 N.D. -0.9 N.D.
nitrogen source Carnitine Hydrochloride (N) -0.0 -3.1 N.D. -1.3 N.D.
pH Minimal media pH8 -0.1 -3.9 N.D. -0.4 N.D.
nitrogen source nitrite (N) -0.1 -3.8 N.D. -0.4 N.D.
nitrogen source Uridine (N) -0.1 -3.9 N.D. -0.2 N.D.
carbon source Carbon source D-Mannitol 10 mM -0.1 -3.0 N.D. -1.1 N.D.
carbon source Carbon source D-Mannitol 2.5 mM -0.1 -2.9 N.D. -1.1 N.D.
pH Minimal media pH8 -0.1 -3.5 N.D. -0.5 N.D.
carbon source acetate (C) +0.1 -0.9 N.D. -3.3 N.D.
pH Minimal media pH7 -0.3 -3.8 N.D. -0.0 N.D.
carbon source Carbon source D-Mannitol 2.5 mM -0.1 -3.0 N.D. -1.0 N.D.
carbon source m-Inositol (C) +0.0 -3.4 N.D. -0.7 N.D.
pH Minimal media pH6 -0.0 -3.9 N.D. +0.1 N.D.
phage P5ss-5 phage 10-1 dilution SM calcl2 -0.2 +3.7 N.D. -1.5 N.D.
stress Lomefloxacin 0.0005 mM -0.1 +4.5 N.D. -0.0 N.D.
stress Benzalkonium Chloride 0.016 mg/ml +0.2 +2.4 N.D. +2.4 N.D.
stress methylglyoxal 0.016 vol% +0.1 +3.1 N.D. +1.9 N.D.
stress Doxycycline hyclate 0.0001 mg/ml -0.0 +5.4 N.D. -0.1 N.D.
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