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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_15430 and AO356_15435 are separated by 17 nucleotides
AO356_15435 and AO356_15440 are separated by 415 nucleotides
AO356_15440 and AO356_15445 are separated by 71 nucleotides
AO356_15445 and AO356_15450 are separated by 344 nucleotides
AO356_15430: AO356_15430 - thiamine-phosphate pyrophosphorylase, at 3,493,336 to 3,493,962
_15430
AO356_15435: AO356_15435 - hydrogenase, at 3,493,980 to 3,494,777
_15435
AO356_15440: AO356_15440 - histidine kinase, at 3,495,193 to 3,497,577
_15440
AO356_15445: AO356_15445 - acyl-CoA dehydrogenase, at 3,497,649 to 3,499,298
_15445
AO356_15450: AO356_15450 - AMP nucleosidase, at 3,499,643 to 3,501,106
_15450
Group
Condition
AO356
_15430
AO356
_15435
AO356
_15440
AO356
_15445
AO356
_15450
carbon source
Inosine (C)
-0.5
-1.0
+0.6
-0.2
-2.9
nitrogen source
Inosine (N)
+0.7
-0.2
+0.5
-0.1
-4.3
carbon source
L-Histidine (C)
-0.0
-0.4
+1.5
-0.4
-4.0
carbon source
L-Histidine (C)
-0.2
-0.3
+1.6
-0.4
-3.8
pH
Minimal media pH9
-1.7
-1.4
+0.1
-0.0
+0.1
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.5
-0.2
+0.3
-1.0
-1.3
carbon source
L-Alanine (C)
-0.3
-0.4
-0.1
+0.0
-1.8
carbon source
L-Isoleucine (C)
-1.0
-1.0
+0.3
+0.1
-0.8
nitrogen source
Uridine (N)
-0.1
-0.1
+0.3
+0.0
-2.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.3
-1.6
+0.1
+0.1
-0.2
nitrogen source
D-Serine (N)
-0.4
+0.2
-1.1
+0.1
-0.2
carbon source
L-Leucine (C)
-0.8
-1.3
+0.6
+0.3
-0.2
carbon source
m-Inositol (C)
+0.0
-0.1
+0.6
-0.1
-1.6
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.3
-0.0
+0.6
-0.7
-1.2
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.2
-0.2
+0.5
-0.4
-1.1
stress
copper (II) chloride 2.5 mM
+0.4
-0.7
+0.4
+0.2
-1.3
nitrogen source
nitrite (N)
+0.1
-0.5
+0.3
+0.0
-0.8
carbon source
D-Trehalose (C)
+0.5
-0.3
-0.9
-0.1
-0.1
nitrogen source
Carnitine Hydrochloride (N)
+0.1
-0.5
+0.4
+0.1
-0.9
carbon source
m-Inositol (C)
+0.3
+0.1
+0.6
-0.2
-1.6
stress
Benzalkonium Chloride 0.016 mg/ml
+0.2
+0.3
-0.4
+0.1
-1.0
motility
outer cut, LB soft agar motility assay
-0.4
-0.8
+0.8
-0.1
-0.1
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.2
-0.1
+0.6
-0.2
-1.0
nitrogen source
L-Leucine (N)
-0.5
-0.6
+0.5
+0.3
+0.1
resistance
20 mM putrescine
+0.3
-0.1
+0.7
+0.1
-0.7
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
+0.3
-0.2
+0.7
+0.2
-0.5
motility
outer cut, LB soft agar motility assay
-0.2
-0.3
+0.9
+0.1
+0.3
carbon source
L-Lysine (C)
-0.4
+0.2
+1.1
+0.1
-0.1
stress
nitrate 200 mM
-0.3
+0.5
+0.7
+0.1
+0.2
carbon source
Putrescine (C)
+0.3
-0.5
+1.2
+0.5
-0.2
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