Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_14340 and AO356_14345 overlap by 4 nucleotidesAO356_14345 and AO356_14350 are separated by 16 nucleotidesAO356_14350 and AO356_14355 are separated by 130 nucleotidesAO356_14355 and AO356_14360 overlap by 4 nucleotides AO356_14340: AO356_14340 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 3,260,055 to 3,261,044 _14340 AO356_14345: AO356_14345 - molecular chaperone SurA, at 3,261,041 to 3,262,321 _14345 AO356_14350: AO356_14350 - LPS biosynthesis protein, at 3,262,338 to 3,265,136 _14350 AO356_14355: AO356_14355 - aminoglycoside phosphotransferase, at 3,265,267 to 3,266,292 _14355 AO356_14360: AO356_14360 - mannose-1-phosphate guanylyltransferase, at 3,266,289 to 3,266,960 _14360
Group Condition AO356_14340 AO356_14345 AO356_14350 AO356_14355 AO356_14360
survival 4C survival; 360 hrs N.D. N.D. N.D. -1.9 -2.7
survival 4C survival; 192 hrs N.D. N.D. N.D. -2.3 -2.0
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -1.7 -2.2
survival 4C survival; 1152 hrs N.D. N.D. N.D. -1.2 -2.5
carbon source Glycerol (C) N.D. N.D. N.D. +0.4 -4.0
carbon source Glycerol (C) N.D. N.D. N.D. +0.5 -4.0
carbon source L-Histidine (C) N.D. N.D. N.D. -0.6 -2.9
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -1.6 -1.7
survival 4C survival; 672 hrs N.D. N.D. N.D. -1.2 -2.0
anaerobic Nitrate reduction with yeast extract N.D. N.D. N.D. -1.7 -1.5
pH Minimal media pH9 N.D. N.D. N.D. -1.3 -1.8
carbon source L-Histidine (C) N.D. N.D. N.D. -0.7 -2.1
carbon source L-Lysine (C) N.D. N.D. N.D. -1.1 -1.4
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -0.9 -1.6
pH Minimal media pH8 N.D. N.D. N.D. -1.2 -1.2
pH Minimal media pH8 N.D. N.D. N.D. -0.9 -1.2
carbon source L-Lysine (C) N.D. N.D. N.D. -0.9 -1.2
carbon source D-Alanine (C) N.D. N.D. N.D. -0.2 -1.8
pH Minimal media pH6 N.D. N.D. N.D. -1.1 -0.9
carbon source NAG (C) N.D. N.D. N.D. -0.2 -1.8
pH Minimal media pH6 N.D. N.D. N.D. -0.9 -0.8
carbon source Carbon source Sucrose 2.5 mM N.D. N.D. N.D. -1.7 +0.6
carbon source Carbon source Sucrose 2.5 mM N.D. N.D. N.D. -1.7 +1.3
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. N.D. N.D. -1.2 +1.0
carbon source Carbon source D-Mannitol 2.5 mM N.D. N.D. N.D. -0.4 +1.3
carbon source D-Xylose (C) N.D. N.D. N.D. +1.1 +0.7
carbon source D-Xylose (C) N.D. N.D. N.D. +1.1 +0.7
stress Rifampicin 0.0024 mg/ml N.D. N.D. N.D. +0.9 +1.0
stress Ceftazidime 0.2 mg/ml N.D. N.D. N.D. +1.1 +1.0
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. N.D. N.D. +1.0 +1.8
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