Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_14265 and AO356_14270 are separated by 67 nucleotidesAO356_14270 and AO356_14275 are separated by 478 nucleotidesAO356_14275 and AO356_14280 are separated by 199 nucleotidesAO356_14280 and AO356_14285 are separated by 8 nucleotides AO356_14265: AO356_14265 - RNA polymerase subunit sigma-70, at 3,243,589 to 3,245,436 _14265 AO356_14270: AO356_14270 - DNA primase, at 3,245,504 to 3,247,462 _14270 AO356_14275: AO356_14275 - 30S ribosomal protein S21, at 3,247,941 to 3,248,156 _14275 AO356_14280: AO356_14280 - tRNA threonylcarbamoyl adenosine modification protein TsaD, at 3,248,356 to 3,249,381 _14280 AO356_14285: AO356_14285 - glycerol-3-phosphate acyltransferase, at 3,249,390 to 3,249,959 _14285
Group Condition AO356_14265 AO356_14270 AO356_14275 AO356_14280 AO356_14285
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. N.D. -1.0
stress 1-ethyl-3-methylimidazolium acetate 20 mM N.D. N.D. N.D. N.D. -0.9
carbon source L-Arabinose (C) N.D. N.D. N.D. N.D. -0.8
pH Minimal media pH8 N.D. N.D. N.D. N.D. -0.8
carbon source Carnitine Hydrochloride (C) N.D. N.D. N.D. N.D. -0.7
carbon source L-Asparagine (C) N.D. N.D. N.D. N.D. -0.7
stress syringaldehyde 10 mM N.D. N.D. N.D. N.D. -0.7
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. N.D. N.D. N.D. -0.6
stress Cephalothin 2 mg/ml N.D. N.D. N.D. N.D. -0.6
carbon source L-Asparagine (C) N.D. N.D. N.D. N.D. -0.6
survival 4C survival; 1152 hrs N.D. N.D. N.D. N.D. -0.6
carbon source D-Glucose (C) N.D. N.D. N.D. N.D. -0.6
pH Minimal media pH7 N.D. N.D. N.D. N.D. -0.6
carbon source NAG (C) N.D. N.D. N.D. N.D. -0.6
stress KB-half, Pseudo5 supernatant N.D. N.D. N.D. N.D. -0.6
carbon source L-Malic (C) N.D. N.D. N.D. N.D. -0.6
carbon source D-Lactate (C) N.D. N.D. N.D. N.D. +0.6
stress Cisplatin 0.0032 mg/ml N.D. N.D. N.D. N.D. +0.6
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. N.D. N.D. N.D. +0.7
nitrogen source Urea (N) N.D. N.D. N.D. N.D. +0.7
stress Cisplatin 0.0032 mg/ml N.D. N.D. N.D. N.D. +0.7
carbon source D-Gluconic Acid (C) N.D. N.D. N.D. N.D. +0.7
stress perchlorate 75 mM N.D. N.D. N.D. N.D. +0.7
stress Rifampicin 0.0024 mg/ml N.D. N.D. N.D. N.D. +0.7
stress Nickel (II) chloride 0.625 mM N.D. N.D. N.D. N.D. +0.8
nitrogen source L-Phenylalanine (N) N.D. N.D. N.D. N.D. +0.9
stress Doxycycline hyclate 0.0001 mg/ml N.D. N.D. N.D. N.D. +0.9
stress Cobalt chloride 0.08 mM N.D. N.D. N.D. N.D. +1.1
stress Nickel (II) chloride 0.625 mM N.D. N.D. N.D. N.D. +1.5
stress Cobalt chloride 0.08 mM N.D. N.D. N.D. N.D. +1.8
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