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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_13490 and AO356_13495 are separated by 158 nucleotides
AO356_13495 and AO356_13500 are separated by 186 nucleotides
AO356_13500 and AO356_13505 overlap by 8 nucleotides
AO356_13505 and AO356_13510 are separated by 229 nucleotides
AO356_13490: AO356_13490 - protein PilI, at 3,055,415 to 3,055,954
_13490
AO356_13495: AO356_13495 - chemotaxis protein, at 3,056,113 to 3,058,176
_13495
AO356_13500: AO356_13500 - histidine kinase, at 3,058,363 to 3,064,299
_13500
AO356_13505: AO356_13505 - chemotaxis protein, at 3,064,292 to 3,064,771
_13505
AO356_13510: AO356_13510 - malonate decarboxylase subunit alpha, at 3,065,001 to 3,066,671
_13510
Group
Condition
AO356
_13490
AO356
_13495
AO356
_13500
AO356
_13505
AO356
_13510
pH
Minimal media pH6
-0.7
-0.4
-0.6
-2.7
+0.1
stress
Sisomicin 0.001 mg/ml
-1.1
+0.3
-1.4
-1.3
-0.2
carbon source
L-Histidine (C)
-1.2
-0.5
-0.6
-1.1
-0.1
carbon source
L-Histidine (C)
-1.0
-0.5
-0.6
-0.9
-0.1
pH
Minimal media pH8
-0.5
-0.4
-0.3
-2.1
+0.0
stress
acetate 40 mM
-0.7
-0.1
-0.3
-1.5
-0.1
temperature
LB 37C
-0.8
-0.4
-0.4
-0.7
-0.1
stress
1-ethyl-3-methylimidazolium acetate 20 mM
-0.3
-0.1
-0.3
-1.5
-0.1
pH
Minimal media pH6
-0.5
-0.6
-0.5
-0.4
-0.0
carbon source
Carbon source Sucrose 2.5 mM
-0.8
-0.0
-0.1
-1.1
-0.0
stress
Spectinomycin 0.025 mg/ml
-0.4
-0.3
-0.3
-1.0
-0.0
stress
Cholin acetate 40 mM
-1.0
-0.3
-0.4
-0.3
-0.1
survival
4C survival; 192 hrs
-1.0
-0.2
-0.3
-0.3
-0.1
carbon source
Tween 20 (C)
-0.1
-0.2
-0.2
-1.3
-0.0
stress
Cisplatin 0.0032 mg/ml
-0.8
+0.0
-0.5
-0.7
+0.1
stress
Cholin acetate 20 mM
-0.3
-0.4
-0.3
-0.7
+0.0
carbon source
L-Lysine (C)
-0.5
-0.6
-0.1
-0.4
+0.1
stress
Cisplatin 0.0032 mg/ml
+0.2
-0.4
-0.3
-1.0
-0.0
stress
1-ethyl-3-methylimidazolium acetate 10 mM
-0.2
-0.2
-0.3
-0.7
-0.0
carbon source
L-Leucine (C)
-1.0
+0.0
-0.3
-0.2
+0.0
carbon source
L-Asparagine (C)
-0.6
-0.3
-0.2
-0.2
+0.1
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.2
-0.4
+0.1
-0.7
-0.2
stress
KB-half, Pseudo5 supernatant
-0.7
+0.2
-0.0
-0.6
+0.2
nitrogen source
L-Isoleucine (N)
+0.2
+0.8
-0.3
-0.4
+0.1
motility
outer cut, LB soft agar motility assay
+0.6
+0.6
-0.1
+0.3
+0.0
stress
Nickel (II) chloride 0.625 mM
+0.7
+0.1
+0.1
+0.7
+0.0
stress
copper (II) chloride 2.5 mM
+0.8
+0.5
+0.3
+0.5
+0.3
stress
methylglyoxal 0.016 vol%
+0.6
+0.2
+0.8
+1.2
+0.0
motility
outer cut, LB soft agar motility assay
+1.1
+0.7
+0.4
+1.5
-0.1
motility
outer cut, LB soft agar motility assay
+1.7
+1.2
+0.9
+1.1
-0.1
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