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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_13380 and AO356_13385 are separated by 145 nucleotides
AO356_13385 and AO356_13390 are separated by 18 nucleotides
AO356_13390 and AO356_13395 overlap by 4 nucleotides
AO356_13395 and AO356_13400 overlap by 4 nucleotides
AO356_13380: AO356_13380 - tRNA (guanine-N7)-methyltransferase, at 3,038,085 to 3,038,810
_13380
AO356_13385: AO356_13385 - hypothetical protein, at 3,038,956 to 3,039,279
_13385
AO356_13390: AO356_13390 - coproporphyrinogen III oxidase, at 3,039,298 to 3,040,497
_13390
AO356_13395: AO356_13395 - non-canonical purine NTP pyrophosphatase, at 3,040,494 to 3,041,090
_13395
AO356_13400: AO356_13400 - homoserine acetyltransferase, at 3,041,087 to 3,041,521
_13400
Group
Condition
AO356
_13380
AO356
_13385
AO356
_13390
AO356
_13395
AO356
_13400
phage
P5ss-5 phage 10-1 dilution SM calcl2
-2.8
+0.4
-0.5
-3.8
-0.1
motility
outer cut, LB soft agar motility assay
-0.4
+0.2
-0.3
-4.6
-0.1
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-1.7
-0.3
-0.5
-0.8
-0.0
carbon source
Glycerol (C)
+0.1
-0.2
+0.1
-3.3
+0.1
carbon source
D-Xylose (C)
+0.7
-0.0
+0.2
-3.4
-0.1
carbon source
Carbon source D,L-Lactate 10 mM
+0.2
+0.1
-0.2
-2.7
+0.1
stress
Cobalt chloride 0.08 mM
+1.1
-0.2
-0.1
-3.3
+0.2
carbon source
acetate (C)
-0.8
-0.0
-0.2
-1.1
-0.1
carbon source
L-Asparagine (C)
-0.3
+0.0
-0.4
-1.7
+0.1
carbon source
succinate (C)
-0.3
+0.0
-0.0
-1.7
-0.0
carbon source
L-Lysine (C)
+0.1
-0.4
-0.1
-1.4
-0.1
carbon source
acetate (C)
-0.8
+0.2
+0.0
-1.1
-0.2
carbon source
L-Isoleucine (C)
+0.1
+0.1
+0.0
-2.0
+0.1
carbon source
L-Arabinose (C)
+0.1
+0.0
-0.1
-1.8
+0.1
stress
KB-half, Pseudo5 supernatant
-0.2
+0.1
-0.0
-1.6
+0.4
stress
Chloramphenicol 0.005 mg/ml
+0.5
-0.2
-0.1
-1.5
+0.1
motility
outer cut, LB soft agar motility assay
+0.6
-0.1
-0.5
-1.3
+0.2
stress
copper (II) chloride 2.5 mM
+0.4
-0.2
+0.4
-1.0
-0.3
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.1
-0.1
+0.4
-1.3
+0.3
carbon source
NAG (C)
+0.8
+0.1
-0.1
-1.3
-0.0
stress
Paraquat dichloride 0.0625 mg/ml
+0.5
-0.1
+0.3
-1.1
-0.1
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+1.4
-0.4
-0.5
-0.1
+0.1
stress
Nickel (II) chloride 0.625 mM
+1.4
-0.1
+0.1
-0.8
-0.0
motility
outer cut, LB soft agar motility assay
+0.6
-0.1
-0.6
+0.9
-0.0
stress
Benzalkonium Chloride 0.016 mg/ml
+0.1
+0.6
+0.7
-0.4
+0.2
stress
Chlorite 0.08 mM
+0.7
-0.5
+0.7
+0.7
+0.3
stress
Spectinomycin 0.0125 mg/ml
+0.3
+0.1
+0.6
+1.0
+0.2
stress
Lomefloxacin 0.0005 mM
+0.4
+0.0
+1.1
+1.1
+0.2
stress
Doxycycline hyclate 0.0001 mg/ml
+0.9
+0.1
+0.5
+0.9
+0.4
stress
Choline chloride 500 mM
+0.3
+0.6
+0.6
+1.2
+0.1
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