Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_13345 and AO356_13350 overlap by 4 nucleotidesAO356_13350 and AO356_13355 are separated by 113 nucleotidesAO356_13355 and AO356_13360 are separated by 197 nucleotidesAO356_13360 and AO356_13365 are separated by 73 nucleotides AO356_13345: AO356_13345 - cell division ATP-binding protein FtsE, at 3,032,912 to 3,033,583 _13345 AO356_13350: AO356_13350 - cell division protein FtsX, at 3,033,580 to 3,034,602 _13350 AO356_13355: AO356_13355 - RNA polymerase subunit sigma-70, at 3,034,716 to 3,035,570 _13355 AO356_13360: AO356_13360 - monofunctional biosynthetic peptidoglycan transglycosylase, at 3,035,768 to 3,036,490 _13360 AO356_13365: AO356_13365 - hypothetical protein, at 3,036,564 to 3,036,935 _13365
Group Condition AO356_13345 AO356_13350 AO356_13355 AO356_13360 AO356_13365
stress Bacitracin 2 mg/ml -7.3 -5.3 N.D. +0.1 -0.2
stress Benzalkonium Chloride 0.016 mg/ml -4.7 -3.8 N.D. +0.0 +0.1
stress Sisomicin 0.001 mg/ml -3.3 -3.6 N.D. -0.7 +0.3
stress Benzethonium chloride 0.025 mM -3.3 -3.7 N.D. -0.2 +0.1
stress Benzalkonium Chloride 0.008 mg/ml -3.6 -2.9 N.D. -0.1 -0.0
stress Rifampicin 0.0024 mg/ml -3.5 -3.2 N.D. +0.1 +0.1
stress Rifampicin 0.0016 mg/ml -2.6 -2.0 N.D. -0.1 -0.1
pH Minimal media pH9 -2.8 -1.7 N.D. -0.4 +0.1
motility outer cut, LB soft agar motility assay -2.9 -1.7 N.D. +0.3 -0.3
stress 1-ethyl-3-methylimidazolium lysine 10 mM -2.3 -2.0 N.D. -0.0 -0.2
stress nitrate 200 mM -2.7 -1.9 N.D. +0.3 -0.0
stress Cholin acetate 40 mM -2.1 -1.8 N.D. -0.4 +0.1
survival 4C survival; 192 hrs -2.1 -1.4 N.D. +0.1 -0.1
survival 4C survival; 1152 hrs -1.5 -1.7 N.D. -0.0 -0.2
stress Cobalt chloride 0.08 mM -2.0 -1.0 N.D. +0.1 -0.2
anaerobic Nitrate reduction with yeast extract -1.8 -0.9 N.D. -0.5 +0.2
stress Paraquat dichloride 0.0625 mg/ml -1.4 -0.9 N.D. -0.5 +0.0
stress copper (II) chloride 2.5 mM -2.0 -0.6 N.D. -0.0 -0.1
stress Paraquat dichloride 0.0625 mg/ml -1.3 -1.2 N.D. -0.0 -0.0
motility outer cut, LB soft agar motility assay -0.6 -1.5 N.D. -0.2 -0.2
motility outer cut, LB soft agar motility assay -1.5 -1.3 N.D. +0.4 -0.1
stress Cobalt chloride 0.08 mM -1.3 -1.1 N.D. -0.1 -0.0
stress acetate 40 mM -1.3 -1.4 N.D. +0.1 +0.1
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -1.2 -1.3 N.D. +0.2 -0.0
carbon source L-Lysine (C) -1.2 -0.9 N.D. +0.2 -0.4
stress 1-ethyl-3-methylimidazolium acetate 10 mM -1.2 -1.1 N.D. +0.1 +0.1
stress Cholin acetate 20 mM -1.2 -1.0 N.D. +0.5 -0.0
phage P5ss-5 phage 10-1 dilution SM calcl2 -1.2 -1.0 N.D. +0.0 +0.3
motility outer cut, LB soft agar motility assay -1.0 -1.3 N.D. +0.6 +0.1
stress Ceftazidime 0.2 mg/ml -1.4 -0.6 N.D. +0.3 +0.7
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