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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_13320 and AO356_13325 overlap by 1 nucleotides
AO356_13325 and AO356_13330 overlap by 8 nucleotides
AO356_13330 and AO356_13340 are separated by 343 nucleotides
AO356_13340 and AO356_13345 overlap by 4 nucleotides
AO356_13320: AO356_13320 - 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD, at 3,027,689 to 3,028,300
_13320
AO356_13325: AO356_13325 - peptidase M16, at 3,028,300 to 3,029,790
_13325
AO356_13330: AO356_13330 - peptidase M16, at 3,029,783 to 3,031,138
_13330
AO356_13340: AO356_13340 - signal recognition particle-docking protein FtsY, at 3,031,482 to 3,032,915
_13340
AO356_13345: AO356_13345 - cell division ATP-binding protein FtsE, at 3,032,912 to 3,033,583
_13345
Group
Condition
AO356
_13320
AO356
_13325
AO356
_13330
AO356
_13340
AO356
_13345
stress
Bacitracin 2 mg/ml
-0.6
+0.4
+0.6
N.D.
-7.3
motility
outer cut, LB soft agar motility assay
-2.3
-0.6
-0.2
N.D.
-2.9
stress
nitrate 200 mM
-2.2
-0.2
-0.6
N.D.
-2.7
stress
Sisomicin 0.001 mg/ml
-1.3
-0.2
-0.1
N.D.
-3.3
anaerobic
Nitrate reduction with yeast extract
-2.0
-0.4
-0.5
N.D.
-1.8
pH
Minimal media pH9
-1.3
-0.6
-0.2
N.D.
-2.8
survival
4C survival; 192 hrs
-1.2
-0.6
-0.5
N.D.
-2.1
motility
outer cut, LB soft agar motility assay
-2.5
-0.2
-0.2
N.D.
-1.5
carbon source
L-Histidine (C)
-2.3
-0.6
-0.6
N.D.
-0.5
carbon source
L-Histidine (C)
-1.9
-0.6
-0.7
N.D.
-0.4
motility
outer cut, LB soft agar motility assay
-1.8
-0.6
-0.6
N.D.
-0.6
motility
outer cut, LB soft agar motility assay
-1.5
-0.4
-0.5
N.D.
-1.0
survival
4C survival; 1152 hrs
-1.4
-0.3
-0.0
N.D.
-1.5
stress
1-ethyl-3-methylimidazolium lysine 10 mM
-0.5
-0.2
-0.1
N.D.
-2.3
stress
Choline chloride 500 mM
-0.6
-0.5
-0.7
N.D.
-1.1
stress
Chlorite 0.08 mM
-2.0
-0.3
-0.3
N.D.
-0.2
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.9
-0.3
-0.5
N.D.
-1.0
stress
Cobalt chloride 0.08 mM
-1.3
+0.0
-0.1
N.D.
-1.3
stress
Benzethonium chloride 0.025 mM
+0.1
+0.4
+0.4
N.D.
-3.3
stress
Lomefloxacin 0.0005 mM
-1.3
-0.6
-0.7
N.D.
+0.2
stress
Benzalkonium Chloride 0.008 mg/ml
+0.4
+0.3
+0.6
N.D.
-3.6
stress
Cholin acetate 40 mM
-0.5
+0.3
+0.3
N.D.
-2.1
stress
Rifampicin 0.0016 mg/ml
+0.1
+0.2
+0.4
N.D.
-2.6
stress
Rifampicin 0.0024 mg/ml
+0.9
+0.6
+1.3
N.D.
-3.5
stress
Benzalkonium Chloride 0.016 mg/ml
+1.4
+1.3
+1.5
N.D.
-4.7
carbon source
Ethanol (C)
+1.3
-0.6
-0.6
N.D.
-0.2
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.3
+0.6
+0.7
N.D.
-1.2
stress
Paraquat dichloride 0.0625 mg/ml
+0.5
+0.5
+0.7
N.D.
-1.3
stress
Paraquat dichloride 0.0625 mg/ml
+0.9
+0.5
+0.6
N.D.
-1.4
stress
methylglyoxal 0.016 vol%
+1.0
+0.3
+0.4
N.D.
-1.0
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