Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_13215 and AO356_13220 are separated by 2 nucleotidesAO356_13220 and AO356_13225 are separated by 179 nucleotidesAO356_13225 and AO356_13230 overlap by 8 nucleotidesAO356_13230 and AO356_13235 are separated by 86 nucleotides AO356_13215: AO356_13215 - 2OG-Fe(II) oxygenase, at 3,005,059 to 3,005,691 _13215 AO356_13220: AO356_13220 - purine nucleoside phosphorylase, at 3,005,694 to 3,006,185 _13220 AO356_13225: AO356_13225 - GTPase SAR1, at 3,006,365 to 3,007,738 _13225 AO356_13230: AO356_13230 - hypothetical protein, at 3,007,731 to 3,009,098 _13230 AO356_13235: AO356_13235 - diacylglycerol kinase, at 3,009,185 to 3,009,697 _13235
Group Condition AO356_13215 AO356_13220 AO356_13225 AO356_13230 AO356_13235
motility outer cut, LB soft agar motility assay -0.2 -0.9 -0.0 -0.3 N.D.
stress 1-ethyl-3-methylimidazolium chloride 200 mM -0.3 -0.5 -0.4 -0.1 N.D.
stress Chlorite 0.08 mM -0.2 -0.6 +0.1 -0.2 N.D.
stress Cholin acetate 40 mM -0.2 -0.6 -0.2 +0.0 N.D.
stress perchlorate 75 mM -0.0 -0.2 -0.2 -0.3 N.D.
stress Cephalothin 2 mg/ml -0.2 -0.4 +0.1 -0.1 N.D.
temperature LB 20C +0.1 -0.3 -0.2 -0.2 N.D.
stress Tetracycline 0.0001 mg/ml -0.4 -0.2 -0.0 +0.1 N.D.
stress KB-half, Pseudo11 supernatant, 100 uM FeCl3 +0.1 -0.4 +0.3 -0.4 N.D.
stress methylglyoxal 0.016 vol% +0.0 -0.4 +0.1 -0.3 N.D.
stress Sisomicin 0.001 mg/ml +0.1 -0.2 +0.2 -0.5 N.D.
stress 1-ethyl-3-methylimidazolium acetate 10 mM -0.3 -0.3 -0.0 +0.3 N.D.
carbon source L-Lysine (C) -0.2 -0.2 +0.3 -0.3 N.D.
stress Benzalkonium Chloride 0.008 mg/ml -0.1 +0.2 +0.1 -0.4 N.D.
carbon source D-Gluconic Acid (C) +0.0 -0.5 +0.2 +0.2 N.D.
survival 4C survival; 1152 hrs +0.0 -0.3 +0.3 -0.1 N.D.
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +0.2 -0.0 +0.1 -0.4 N.D.
stress copper (II) chloride 2.5 mM +0.5 -0.5 +0.3 -0.1 N.D.
survival 4C survival; 672 hrs +0.2 -0.3 +0.2 +0.1 N.D.
stress nitrate 200 mM +0.3 +0.0 +0.1 -0.3 N.D.
stress syringaldehyde 10 mM -0.2 -0.2 +0.3 +0.3 N.D.
anaerobic Nitrate reduction with yeast extract +0.6 -0.0 +0.1 -0.5 N.D.
carbon source Carbon source Sucrose 2.5 mM +0.1 -0.2 +0.2 +0.2 N.D.
stress KB-half, Pseudo5 supernatant +0.4 -0.4 +0.5 -0.2 N.D.
stress D-Cycloserine 0.1 mg/ml -0.2 +0.1 +0.2 +0.4 N.D.
motility outer cut, LB soft agar motility assay -0.1 -0.2 +0.6 +0.0 N.D.
carbon source Putrescine (C) +0.5 +0.3 -0.1 -0.1 N.D.
nitrogen source Inosine (N) -0.2 +0.4 +0.2 +0.1 N.D.
phage P5ss-5 phage 10-1 dilution SM calcl2 +0.4 -0.2 +0.1 +0.4 N.D.
pH Minimal media pH8 +0.2 +0.3 +0.2 +0.1 N.D.
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