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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_13135 and AO356_13140 are separated by 278 nucleotides
AO356_13140 and AO356_13145 are separated by 40 nucleotides
AO356_13145 and AO356_13150 are separated by 58 nucleotides
AO356_13150 and AO356_13155 are separated by 181 nucleotides
AO356_13135: AO356_13135 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)
(from data)
, at 2,986,014 to 2,987,390
_13135
AO356_13140: AO356_13140 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94)
(from data)
, at 2,987,669 to 2,988,445
_13140
AO356_13145: AO356_13145 - glutamine synthetase, at 2,988,486 to 2,989,844
_13145
AO356_13150: AO356_13150 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)
(from data)
, at 2,989,903 to 2,991,267
_13150
AO356_13155: AO356_13155 - spermidine/putrescine ABC transporter substrate-binding protein, at 2,991,449 to 2,992,546
_13155
Group
Condition
AO356
_13135
AO356
_13140
AO356
_13145
AO356
_13150
AO356
_13155
carbon source
Putrescine (C)
-6.0
-2.0
-4.3
-4.4
-5.0
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-3.4
-1.6
-3.5
-3.1
-3.6
nitrogen source
Putrescine (N)
-5.5
+0.4
-3.1
-3.2
-3.9
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-4.1
-1.6
-3.2
-2.9
-3.3
resistance
20 mM putrescine
-3.4
-1.9
-3.3
-2.8
-3.6
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-3.5
-1.5
-3.4
-2.8
-3.2
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-2.5
-1.4
-3.6
-3.0
-3.1
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-2.8
-1.4
-3.3
-2.8
-3.3
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-2.3
-0.9
-3.6
-2.4
-3.1
nitrogen source
D-Serine (N)
-0.2
+0.2
-0.8
-0.5
-0.5
stress
Cholin acetate 40 mM
-0.3
+0.2
-1.6
-0.1
-0.0
carbon source
acetate (C)
+0.6
-0.5
-0.4
-0.2
-1.2
anaerobic
Nitrate reduction with yeast extract
-0.2
-0.2
-0.7
-0.3
-0.2
carbon source
acetate (C)
+0.6
-0.5
-0.4
-0.1
-0.9
motility
outer cut, LB soft agar motility assay
-0.2
+0.1
-1.0
-0.2
+0.1
survival
4C survival; 360 hrs
+0.6
-0.3
-0.8
-0.6
+0.1
motility
outer cut, LB soft agar motility assay
-0.1
-0.2
-0.8
-0.2
+0.3
motility
outer cut, LB soft agar motility assay
-0.4
+0.1
-0.8
-0.3
+0.4
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.4
-0.1
-0.7
-0.2
-0.2
carbon source
D-Xylose (C)
+0.2
-0.2
+0.4
-0.0
-0.9
carbon source
L-Histidine (C)
-0.2
-0.1
+0.6
-0.0
+0.7
carbon source
L-Histidine (C)
-0.2
-0.3
+0.9
-0.0
+0.7
stress
Sisomicin 0.001 mg/ml
+0.2
+0.2
+0.8
-0.1
-0.1
stress
Paraquat dichloride 0.0625 mg/ml
-0.1
+1.0
+0.3
+0.3
-0.3
carbon source
D-Alanine (C)
-0.2
+0.2
+0.5
+0.2
+0.5
stress
Lomefloxacin 0.0005 mM
+0.6
+0.2
+0.6
+0.2
-0.3
stress
Benzalkonium Chloride 0.016 mg/ml
-0.0
+0.1
+1.1
+0.3
-0.0
carbon source
Fumarate (C)
+0.4
+0.2
+1.0
+0.0
-0.2
carbon source
L-Lysine (C)
+0.4
+1.6
+0.8
+0.8
-1.6
carbon source
L-Lysine (C)
+0.3
+1.9
+0.8
+0.9
-1.9
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