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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_12935 and AO356_12940 are separated by 124 nucleotides
AO356_12940 and AO356_12945 overlap by 1 nucleotides
AO356_12945 and AO356_12950 are separated by 323 nucleotides
AO356_12950 and AO356_12955 overlap by 25 nucleotides
AO356_12935: AO356_12935 - transcription termination factor Rho, at 2,943,675 to 2,944,934
_12935
AO356_12940: AO356_12940 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 2,945,059 to 2,946,525
_12940
AO356_12945: AO356_12945 - CDP-6-deoxy-delta-3,4-glucoseen reductase, at 2,946,525 to 2,947,493
_12945
AO356_12950: AO356_12950 - calcium transporter ChaC, at 2,947,817 to 2,948,485
_12950
AO356_12955: AO356_12955 - glycerol-3-phosphate transporter, at 2,948,461 to 2,949,810
_12955
Group
Condition
AO356
_12935
AO356
_12940
AO356
_12945
AO356
_12950
AO356
_12955
carbon source
L-Proline (C)
N.D.
N.D.
-4.7
-0.2
+0.0
carbon source
L-Proline (C)
N.D.
N.D.
-3.4
-0.2
+0.1
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
-2.3
+0.2
+0.1
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
-2.3
+0.2
+0.2
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
-2.2
+0.2
+0.2
carbon source
acetate (C)
N.D.
N.D.
-1.6
-0.2
+0.0
stress
Chlorite 0.08 mM
N.D.
N.D.
-1.3
-0.2
-0.2
carbon source
D-Lactate (C)
N.D.
N.D.
-1.6
-0.1
+0.0
stress
1-ethyl-3-methylimidazolium chloride 200 mM
N.D.
N.D.
-1.6
+0.3
-0.3
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
-1.6
+0.0
+0.1
stress
Chlorite 0.04 mM
N.D.
N.D.
-0.7
-0.3
-0.4
carbon source
D-Lactate (C)
N.D.
N.D.
-1.5
+0.0
+0.0
temperature
LB 10C
N.D.
N.D.
-1.5
+0.2
+0.0
stress
Nickel (II) chloride 0.625 mM
N.D.
N.D.
-1.2
-0.1
-0.0
stress
Cholin acetate 40 mM
N.D.
N.D.
-0.9
-0.2
+0.1
stress
methylglyoxal 0.016 vol%
N.D.
N.D.
+0.7
-0.9
+0.1
nitrogen source
L-Isoleucine (N)
N.D.
N.D.
+0.9
-0.2
-0.1
carbon source
L-Isoleucine (C)
N.D.
N.D.
+1.4
-0.2
-0.1
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
N.D.
N.D.
+1.2
+0.1
-0.1
carbon source
L-Lysine (C)
N.D.
N.D.
+1.2
+0.2
+0.0
carbon source
L-Lysine (C)
N.D.
N.D.
+1.0
+0.2
+0.1
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
N.D.
N.D.
+1.6
-0.1
-0.1
stress
Lomefloxacin 0.0005 mM
N.D.
N.D.
+1.2
+0.5
-0.0
stress
Bacitracin 2 mg/ml
N.D.
N.D.
+1.0
+0.2
+0.3
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
N.D.
N.D.
+2.1
-0.0
-0.2
stress
Sisomicin 0.001 mg/ml
N.D.
N.D.
+2.5
-0.5
+0.1
stress
Aluminum chloride 5 mM
N.D.
N.D.
+0.7
+0.2
+1.5
stress
Rifampicin 0.0024 mg/ml
N.D.
N.D.
+2.2
+0.0
+0.1
stress
Benzalkonium Chloride 0.016 mg/ml
N.D.
N.D.
+2.0
+0.1
+0.9
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
N.D.
N.D.
-0.0
+0.3
+4.7
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