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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_12840 and AO356_12845 are separated by 84 nucleotides
AO356_12845 and AO356_12850 are separated by 3 nucleotides
AO356_12850 and AO356_12855 are separated by 154 nucleotides
AO356_12855 and AO356_12860 are separated by 18 nucleotides
AO356_12840: AO356_12840 - threonine transporter RhtB, at 2,924,414 to 2,925,046
_12840
AO356_12845: AO356_12845 - penicillin-binding protein activator LpoB, at 2,925,131 to 2,925,874
_12845
AO356_12850: AO356_12850 - penicillin-binding protein activator LpoB, at 2,925,878 to 2,926,465
_12850
AO356_12855: AO356_12855 - hypothetical protein, at 2,926,620 to 2,926,991
_12855
AO356_12860: AO356_12860 - hypothetical protein, at 2,927,010 to 2,928,404
_12860
Group
Condition
AO356
_12840
AO356
_12845
AO356
_12850
AO356
_12855
AO356
_12860
carbon source
D-Trehalose (C)
-1.0
-0.1
-0.1
-0.1
-0.1
carbon source
D-Trehalose (C)
-1.0
+0.1
-0.1
-0.0
-0.2
nitrogen source
Gly-Glu (N)
-1.0
-0.0
+0.0
-0.0
+0.0
anaerobic
Nitrate reduction with yeast extract
-0.8
+0.2
-0.0
-0.1
-0.1
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-1.0
+0.1
+0.1
+0.1
+0.0
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.8
-0.0
+0.2
+0.1
-0.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.2
-0.3
-0.3
+0.1
+0.1
stress
methylglyoxal 0.016 vol%
-0.3
+0.1
+0.0
-0.2
-0.1
stress
copper (II) chloride 2.5 mM
-0.1
+0.1
-0.2
-0.5
+0.2
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.5
-0.1
+0.1
+0.1
-0.1
stress
perchlorate 75 mM
-0.2
-0.1
-0.1
-0.3
+0.2
motility
outer cut, LB soft agar motility assay
-0.0
-0.2
+0.2
-0.4
-0.0
motility
outer cut, LB soft agar motility assay
-0.5
-0.1
-0.0
+0.4
-0.1
stress
sodium fluoride 6.25 mM
+0.1
-0.0
-0.1
-0.4
+0.1
carbon source
Carbon source D,L-Lactate 10 mM
-0.2
-0.2
+0.2
-0.1
+0.0
carbon source
Carbon source D-Mannitol 5 mM
-0.3
-0.1
-0.1
+0.2
+0.1
stress
Nalidixic 0.004 mg/ml
-0.3
-0.0
+0.1
-0.1
+0.1
pH
Minimal media pH9
+0.2
-0.2
-0.2
+0.2
-0.1
stress
nitrite 10 mM
-0.3
+0.3
+0.1
-0.5
+0.1
stress
Lomefloxacin 0.0005 mM
-0.1
+0.2
+0.0
-0.3
+0.1
nitrogen source
Urea (N)
-0.3
+0.2
+0.1
+0.1
-0.2
stress
Chlorite 0.08 mM
-0.2
-0.2
+0.1
+0.1
+0.2
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.3
+0.2
+0.1
+0.1
-0.1
survival
4C survival; 672 hrs
-0.2
+0.1
+0.3
-0.2
-0.0
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.0
+0.0
+0.1
-0.4
+0.2
stress
Cisplatin 0.0032 mg/ml
+0.0
+0.1
-0.2
-0.1
+0.3
stress
Fusidic 0.1 mg/ml
-0.1
+0.2
+0.3
+0.0
+0.1
nitrogen source
L-tyrosine (N)
+0.2
+0.2
+0.2
+0.0
+0.1
stress
1-ethyl-3-methylimidazolium acetate 10 mM
+0.4
-0.0
+0.2
+0.2
+0.1
stress
Doxycycline hyclate 0.0001 mg/ml
-0.0
+0.1
+0.4
+0.1
+0.3
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