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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_12500 and AO356_12505 are separated by 34 nucleotides
AO356_12505 and AO356_12510 are separated by 363 nucleotides
AO356_12510 and AO356_12515 are separated by 95 nucleotides
AO356_12515 and AO356_12520 are separated by 82 nucleotides
AO356_12500: AO356_12500 - ribonuclease PH, at 2,853,219 to 2,853,941
_12500
AO356_12505: AO356_12505 - orotate phosphoribosyltransferase, at 2,853,976 to 2,854,344
_12505
AO356_12510: AO356_12510 - sodium:glutamate symporter, at 2,854,708 to 2,855,916
_12510
AO356_12515: AO356_12515 - exodeoxyribonuclease III, at 2,856,012 to 2,856,791
_12515
AO356_12520: AO356_12520 - orotate phosphoribosyltransferase, at 2,856,874 to 2,857,518
_12520
Group
Condition
AO356
_12500
AO356
_12505
AO356
_12510
AO356
_12515
AO356
_12520
survival
4C survival; 192 hrs
-1.7
+0.0
-0.3
-3.0
-4.4
carbon source
D-Glucosamine Hydrochloride (C)
+0.8
-0.0
+0.0
-3.4
-4.5
stress
Sisomicin 0.001 mg/ml
-0.1
-0.2
-0.2
-2.8
-3.6
stress
Spectinomycin 0.025 mg/ml
-1.2
-0.0
-0.2
-2.3
-3.1
stress
methylglyoxal 0.016 vol%
+0.5
-0.0
-0.3
-3.6
-3.2
survival
4C survival; 1152 hrs
-0.6
+0.1
-0.2
-2.5
-3.5
pH
Minimal media pH9
-1.3
-0.1
-0.2
-0.4
-4.7
motility
outer cut, LB soft agar motility assay
-0.4
-0.1
+0.3
-2.3
-4.2
carbon source
Carbon source Sucrose 10 mM
-0.7
-0.3
+0.1
-1.2
-4.1
nitrogen source
D-Glucosamine Hydrochloride (N)
+0.2
+0.1
-0.0
-1.2
-5.0
carbon source
L-Proline (C)
-0.3
+0.1
+0.2
-1.4
-4.4
pH
Minimal media pH8
-0.4
-0.2
-0.1
+1.0
-4.7
nitrogen source
D-Serine (N)
+1.0
+0.0
+0.3
-1.7
-3.8
nitrogen source
Adenine (N)
+0.9
+0.2
-0.3
+0.3
-5.1
carbon source
Carbon source D-Mannitol 5 mM
-1.1
+0.0
-0.1
+1.3
-4.1
nitrogen source
Inosine (N)
+1.8
+0.0
-0.2
-0.6
-5.0
carbon source
L-Isoleucine (C)
-1.9
-0.0
-0.1
+2.5
-4.3
carbon source
L-Alanine (C)
-0.7
-0.1
+0.2
+1.1
-4.4
nitrogen source
L-Isoleucine (N)
-2.5
-0.2
+0.3
+3.0
-4.3
nitrogen source
L-Phenylalanine (N)
-0.3
-0.2
-0.0
+1.4
-4.4
carbon source
Inosine (C)
+1.0
-0.0
-0.1
+1.0
-4.5
carbon source
Ethanol (C)
+0.4
-0.0
-0.0
+1.7
-4.4
carbon source
L-Leucine (C)
+0.6
-0.0
+0.1
+1.9
-4.3
carbon source
D-Alanine (C)
+0.5
-0.0
+0.1
+2.3
-4.4
carbon source
L-Histidine (C)
+0.5
-0.2
+0.0
+2.6
-4.3
carbon source
D-Fructose (C)
-0.1
-0.1
+0.2
+2.5
-3.5
carbon source
D-Fructose (C)
-0.1
+0.0
+0.1
+2.7
-3.6
carbon source
L-Histidine (C)
+0.4
+0.0
+0.1
+2.5
-3.5
nitrogen source
L-Leucine (N)
-0.3
+0.2
+0.2
+5.5
-4.4
nitrogen source
L-tyrosine (N)
-0.7
-0.2
+0.4
+7.8
-3.9
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