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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_12240 and AO356_12245 are separated by 158 nucleotides
AO356_12245 and AO356_12250 overlap by 8 nucleotides
AO356_12250 and AO356_12255 overlap by 37 nucleotides
AO356_12255 and AO356_12260 overlap by 4 nucleotides
AO356_12240: AO356_12240 - type VI secretion protein ImpA, at 2,791,727 to 2,793,664
_12240
AO356_12245: AO356_12245 - ClpV1 family T6SS ATPase, at 2,793,823 to 2,796,501
_12245
AO356_12250: AO356_12250 - type VI secretion protein, at 2,796,494 to 2,797,546
_12250
AO356_12255: AO356_12255 - type VI secretion system protein ImpG, at 2,797,510 to 2,799,369
_12255
AO356_12260: AO356_12260 - type VI secretion system lysozyme, at 2,799,366 to 2,799,878
_12260
Group
Condition
AO356
_12240
AO356
_12245
AO356
_12250
AO356
_12255
AO356
_12260
motility
outer cut, LB soft agar motility assay
-0.0
-0.3
-0.1
-0.4
N.D.
survival
4C survival; 1152 hrs
-0.2
-0.3
-0.2
-0.1
N.D.
stress
Choline chloride 500 mM
+0.0
-0.4
-0.1
-0.1
N.D.
stress
Benzalkonium Chloride 0.016 mg/ml
+0.0
-0.5
-0.1
+0.0
N.D.
motility
outer cut, LB soft agar motility assay
+0.0
-0.3
-0.4
+0.1
N.D.
stress
syringaldehyde 5 mM
+0.0
-0.2
-0.2
-0.1
N.D.
nitrogen source
L-Isoleucine (N)
+0.1
-0.3
-0.1
-0.1
N.D.
carbon source
L-Leucine (C)
+0.0
-0.4
+0.0
-0.1
N.D.
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.1
-0.3
+0.2
-0.5
N.D.
anaerobic
Nitrate reduction with yeast extract
-0.2
-0.4
+0.0
+0.1
N.D.
nitrogen source
L-Leucine (N)
+0.0
-0.4
-0.1
+0.1
N.D.
stress
Nickel (II) chloride 0.625 mM
+0.1
-0.3
-0.1
-0.1
N.D.
carbon source
Inosine (C)
+0.0
-0.4
+0.1
-0.1
N.D.
carbon source
NAG (C)
+0.1
-0.4
-0.0
-0.1
N.D.
carbon source
Putrescine (C)
+0.1
-0.2
-0.1
+0.1
N.D.
stress
Lomefloxacin 0.0005 mM
+0.0
-0.2
+0.2
-0.1
N.D.
stress
Tetracycline 0.0001 mg/ml
+0.1
-0.2
+0.2
+0.0
N.D.
carbon source
L-Isoleucine (C)
+0.0
-0.2
+0.1
+0.2
N.D.
stress
methylglyoxal 0.016 vol%
-0.1
-0.2
+0.2
+0.2
N.D.
stress
Sisomicin 0.001 mg/ml
+0.2
-0.2
+0.0
+0.1
N.D.
carbon source
L-Lysine (C)
+0.1
+0.2
-0.3
+0.2
N.D.
motility
outer cut, LB soft agar motility assay
+0.3
-0.0
+0.1
-0.2
N.D.
carbon source
L-Malic (C)
+0.1
-0.1
+0.2
+0.1
N.D.
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.1
-0.1
+0.1
+0.4
N.D.
nitrogen source
D-Serine (N)
+0.3
-0.1
+0.1
+0.2
N.D.
stress
Chlorite 0.04 mM
+0.1
-0.0
+0.4
+0.2
N.D.
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.4
+0.0
+0.3
+0.2
N.D.
stress
Paraquat dichloride 0.0625 mg/ml
+0.4
+0.2
+0.2
+0.5
N.D.
stress
Paraquat dichloride 0.0625 mg/ml
+0.4
+0.2
+0.4
+0.2
N.D.
stress
KB-half, Pseudo5 supernatant
+0.1
+0.5
+0.5
+0.5
N.D.
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