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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_11305 and AO356_11310 are separated by 163 nucleotides
AO356_11310 and AO356_11315 are separated by 13 nucleotides
AO356_11315 and AO356_11320 are separated by 112 nucleotides
AO356_11320 and AO356_11325 are separated by 208 nucleotides
AO356_11305: AO356_11305 - quinone oxidoreductase, at 2,562,578 to 2,563,555
_11305
AO356_11310: AO356_11310 - coproporphyrinogen III oxidase, at 2,563,719 to 2,564,633
_11310
AO356_11315: AO356_11315 - shikimate dehydrogenase, at 2,564,647 to 2,565,468
_11315
AO356_11320: AO356_11320 - SulP family inorganic anion transporter, at 2,565,581 to 2,567,149
_11320
AO356_11325: AO356_11325 - glycine/betaine ABC transporter substrate-binding protein, at 2,567,358 to 2,568,278
_11325
Group
Condition
AO356
_11305
AO356
_11310
AO356
_11315
AO356
_11320
AO356
_11325
pH
Minimal media pH6
+0.0
N.D.
-3.8
-0.1
-0.1
pH
Minimal media pH7
-0.0
N.D.
-3.7
-0.1
-0.1
temperature
LB 20C
+0.0
N.D.
-3.7
-0.1
-0.1
pH
Minimal media pH8
-0.0
N.D.
-3.7
-0.1
+0.0
pH
Minimal media pH7
-0.0
N.D.
-3.7
-0.0
+0.1
pH
Minimal media pH9
-0.0
N.D.
-3.4
-0.1
+0.1
survival
4C survival; 24 hrs
+0.1
N.D.
-3.5
+0.0
-0.0
stress
Cephalothin 2 mg/ml
+0.0
N.D.
-3.3
+0.0
-0.1
survival
4C survival; 192 hrs
-0.1
N.D.
-3.1
-0.1
-0.0
stress
perchlorate 75 mM
-0.0
N.D.
-3.0
-0.1
-0.1
nitrogen source
Thymine (N)
-0.0
N.D.
-3.0
-0.0
-0.2
nitrogen source
nitrite (N)
-0.0
N.D.
-3.1
-0.1
-0.0
pH
Minimal media pH8
+0.1
N.D.
-3.3
+0.0
-0.0
carbon source
L-Asparagine (C)
-0.0
N.D.
-3.1
-0.0
-0.1
nitrogen source
Adenosine (N)
+0.0
N.D.
-3.0
-0.0
-0.2
carbon source
Tween 20 (C)
-0.0
N.D.
-2.9
-0.1
-0.1
anaerobic
Nitrate reduction with yeast extract
-0.3
N.D.
-2.8
-0.2
+0.2
nitrogen source
Cytosine (N)
-0.0
N.D.
-3.0
-0.1
-0.0
carbon source
L-Asparagine (C)
+0.0
N.D.
-3.1
-0.0
-0.0
carbon source
L-Glutamine (C)
+0.0
N.D.
-3.0
-0.1
-0.0
nitrogen source
Adenine (N)
+0.0
N.D.
-3.1
+0.1
-0.0
carbon source
L-Arginine (C)
+0.1
N.D.
-3.0
-0.0
-0.1
carbon source
L-Histidine (C)
+0.1
N.D.
-2.9
-0.1
-0.1
pH
Minimal media pH6
+0.0
N.D.
-3.1
+0.1
+0.0
nitrogen source
Ammonium chloride (N)
-0.1
N.D.
-3.0
+0.1
-0.0
survival
4C survival; 360 hrs
-0.0
N.D.
-2.9
-0.1
+0.1
carbon source
L-Aspartic Acid (C)
+0.1
N.D.
-3.0
+0.0
+0.0
nitrogen source
Uridine (N)
+0.1
N.D.
-3.0
-0.0
+0.1
carbon source
Uridine (C)
+0.1
N.D.
-3.0
-0.0
+0.1
carbon source
Tween 20 (C)
+0.2
N.D.
-3.0
+0.0
+0.0
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