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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_10525 and AO356_10535 are separated by 1063 nucleotides
AO356_10535 and AO356_10540 are separated by 11 nucleotides
AO356_10540 and AO356_10545 are separated by 5 nucleotides
AO356_10545 and AO356_10550 are separated by 37 nucleotides
AO356_10525: AO356_10525 - cell division protein, at 2,379,890 to 2,380,378
_10525
AO356_10535: AO356_10535 - ABC transporter permease, at 2,381,442 to 2,382,308
_10535
AO356_10540: AO356_10540 - sulfonate ABC transporter ATP-binding protein, at 2,382,320 to 2,383,180
_10540
AO356_10545: AO356_10545 - nitrate ABC transporter substrate-binding protein, at 2,383,186 to 2,384,211
_10545
AO356_10550: AO356_10550 - taurine dioxygenase, at 2,384,249 to 2,385,151
_10550
Group
Condition
AO356
_10525
AO356
_10535
AO356
_10540
AO356
_10545
AO356
_10550
anaerobic
Nitrate reduction with yeast extract
-0.6
-0.2
-0.7
-0.2
-0.0
motility
outer cut, LB soft agar motility assay
-0.2
-0.2
-1.3
-0.2
+0.2
carbon source
L-Lysine (C)
-0.6
+0.0
-0.5
-0.1
-0.1
carbon source
D-Xylose (C)
-0.3
-0.1
-0.7
-0.0
-0.1
carbon source
L-Aspartic Acid (C)
-0.0
-0.0
-0.7
-0.2
-0.0
carbon source
NAG (C)
-0.2
-0.0
-0.3
-0.2
+0.1
carbon source
Carbon source Sucrose 5 mM
+0.1
-0.0
-0.7
-0.1
+0.1
survival
4C survival; 192 hrs
-0.3
+0.1
-0.2
+0.0
-0.2
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.4
+0.1
-0.3
+0.0
-0.0
carbon source
Carbon source D-Mannitol 10 mM
+0.1
+0.0
-0.6
-0.2
+0.1
stress
nitrite 10 mM
+0.3
+0.1
-0.6
-0.1
-0.1
carbon source
Carbon source Sucrose 10 mM
+0.3
-0.1
-0.4
-0.0
-0.1
carbon source
Carbon source D-Mannitol 10 mM
+0.2
+0.1
-0.3
-0.1
-0.2
carbon source
D-Gluconic Acid (C)
-0.1
-0.2
+0.3
-0.0
-0.2
stress
Chlorite 0.04 mM
+0.2
+0.0
-0.5
+0.0
-0.0
motility
outer cut, LB soft agar motility assay
-0.2
-0.2
+0.3
-0.3
+0.2
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.4
-0.1
-0.2
+0.0
-0.2
stress
Cisplatin 0.0032 mg/ml
+0.2
-0.1
+0.2
-0.2
-0.1
stress
Lomefloxacin 0.0005 mM
-0.3
-0.2
+0.1
+0.2
+0.1
stress
Rifampicin 0.0016 mg/ml
+0.2
+0.1
-0.4
-0.0
+0.1
stress
copper (II) chloride 2.5 mM
-0.1
+0.0
-0.4
+0.3
+0.1
carbon source
Carbon source D-Mannitol 5 mM
+0.1
+0.1
-0.4
-0.0
+0.2
stress
Cholin acetate 40 mM
+0.4
-0.1
-0.1
-0.1
-0.0
resistance
20 mM putrescine
-0.2
-0.2
+0.5
-0.0
+0.1
carbon source
L-Arabinose (C)
+0.1
-0.1
+0.5
-0.1
-0.0
stress
Chloramphenicol 0.005 mg/ml
+0.2
+0.2
-0.2
+0.1
+0.1
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.1
-0.1
+0.3
-0.1
+0.2
pH
Minimal media pH8
+0.1
+0.1
+0.3
+0.2
-0.1
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
+0.1
+0.1
+0.4
+0.1
+0.1
nitrogen source
L-tyrosine (N)
+0.3
+0.2
+0.4
+0.0
-0.1
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