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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_10385 and AO356_10390 overlap by 4 nucleotides
AO356_10390 and AO356_10395 overlap by 4 nucleotides
AO356_10395 and AO356_10400 are separated by 240 nucleotides
AO356_10400 and AO356_10405 are separated by 109 nucleotides
AO356_10385: AO356_10385 - ACR family transporter, at 2,342,515 to 2,345,562
_10385
AO356_10390: AO356_10390 - RND transporter, at 2,345,559 to 2,346,623
_10390
AO356_10395: AO356_10395 - RND transporter, at 2,346,620 to 2,347,723
_10395
AO356_10400: AO356_10400 - ATP-binding protein, at 2,347,964 to 2,349,124
_10400
AO356_10405: AO356_10405 - glutamine ABC transporter substrate-binding protein, at 2,349,234 to 2,350,115
_10405
Group
Condition
AO356
_10385
AO356
_10390
AO356
_10395
AO356
_10400
AO356
_10405
carbon source
L-Lysine (C)
-0.2
-0.2
-0.3
-0.3
-0.1
motility
outer cut, LB soft agar motility assay
-0.0
-0.2
-0.8
-0.1
+0.1
carbon source
L-Aspartic Acid (C)
-0.0
-0.3
-0.3
-0.3
-0.0
stress
Nalidixic 0.004 mg/ml
-0.1
-0.3
-0.2
-0.1
-0.2
stress
Rifampicin 0.0024 mg/ml
-0.0
-0.4
-0.3
-0.1
+0.1
carbon source
Carnitine Hydrochloride (C)
+0.1
+0.1
-0.4
-0.3
-0.1
anaerobic
Nitrate reduction with yeast extract
-0.4
-0.3
-0.6
+0.2
+0.5
stress
Benzethonium chloride 0.025 mM
-0.3
-0.5
+0.0
-0.1
+0.2
temperature
LB 30C
-0.1
-0.3
-0.3
+0.0
+0.2
stress
Cholin acetate 40 mM
+0.1
-0.4
+0.0
+0.1
-0.4
carbon source
L-Malic (C)
-0.0
-0.0
-0.3
-0.3
+0.2
stress
Cisplatin 0.0032 mg/ml
-0.1
+0.2
-0.1
-0.2
-0.4
carbon source
Carbon source D,L-Lactate 10 mM
-0.1
+0.2
-0.3
-0.1
-0.3
motility
outer cut, LB soft agar motility assay
-0.1
+0.1
-0.5
+0.1
-0.2
stress
nitrite 10 mM
-0.1
-0.3
-0.1
-0.2
+0.2
stress
Benzalkonium Chloride 0.016 mg/ml
-0.3
-0.2
-0.4
+0.4
+0.1
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.2
+0.2
-0.4
+0.3
-0.2
stress
copper (II) chloride 2.5 mM
+0.0
-0.1
-0.4
-0.2
+0.4
stress
Nickel (II) chloride 0.625 mM
+0.1
+0.0
-0.3
-0.3
+0.2
stress
Cephalothin 2 mg/ml
-0.0
+0.3
-0.5
+0.1
-0.1
carbon source
Carbon source Sucrose 2.5 mM
+0.0
+0.4
-0.1
-0.3
-0.1
carbon source
L-Proline (C)
+0.1
-0.2
+0.2
-0.4
+0.2
carbon source
pyruvate (C)
+0.1
+0.0
-0.3
-0.2
+0.3
carbon source
Carbon source D-Mannitol 5 mM
+0.0
+0.4
+0.0
-0.2
-0.2
stress
Chlorite 0.08 mM
-0.1
+0.4
-0.1
-0.3
+0.1
carbon source
Tween 20 (C)
-0.0
+0.1
+0.4
-0.3
+0.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.2
+0.1
+0.4
+0.1
-0.1
nitrogen source
Urea (N)
+0.2
+0.1
+0.2
+0.3
+0.0
stress
Sisomicin 0.001 mg/ml
+0.1
-0.3
+0.5
+0.3
+0.3
stress
KB-half, Pseudo5 supernatant
-0.0
+0.7
+0.8
-0.1
-0.0
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