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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_10235 and AO356_10240 overlap by 1 nucleotides
AO356_10240 and AO356_10245 are separated by 3 nucleotides
AO356_10245 and AO356_10250 are separated by 507 nucleotides
AO356_10250 and AO356_10255 are separated by 334 nucleotides
AO356_10235: AO356_10235 - cystine transporter subunit, at 2,310,457 to 2,311,257
_10235
AO356_10240: AO356_10240 - amino acid ABC transporter permease, at 2,311,257 to 2,311,922
_10240
AO356_10245: AO356_10245 - ectoine/hydroxyectoine ABC transporter ATP-binding protein EhuA, at 2,311,926 to 2,312,687
_10245
AO356_10250: AO356_10250 - hypothetical protein, at 2,313,195 to 2,313,965
_10250
AO356_10255: AO356_10255 - SfnB family sulfur acquisition oxidoreductase, at 2,314,300 to 2,315,541
_10255
Group
Condition
AO356
_10235
AO356
_10240
AO356
_10245
AO356
_10250
AO356
_10255
stress
Nickel (II) chloride 0.625 mM
-1.1
-1.2
-1.3
-0.0
-0.2
stress
Cobalt chloride 0.08 mM
-0.8
-1.2
-1.4
-0.2
-0.0
stress
Nickel (II) chloride 0.625 mM
-0.8
-0.9
-1.2
+0.0
-0.1
stress
Cobalt chloride 0.08 mM
-0.8
-1.0
-1.2
+0.1
+0.1
motility
outer cut, LB soft agar motility assay
-0.3
-0.2
-1.3
-0.2
-0.2
stress
Lomefloxacin 0.0005 mM
-0.2
-0.2
-1.1
-0.3
+0.0
stress
Chlorite 0.04 mM
+0.1
-0.1
-1.1
-0.1
-0.4
stress
nitrite 10 mM
-0.4
-0.2
-0.7
+0.1
-0.0
survival
4C survival; 360 hrs
-0.1
-0.1
-0.6
-0.1
-0.3
stress
Cisplatin 0.0032 mg/ml
-0.0
-0.3
-0.5
-0.1
-0.2
pH
Minimal media pH9
-0.3
+0.0
-0.6
-0.0
-0.1
nitrogen source
Carnitine Hydrochloride (N)
-0.2
-0.1
-0.4
-0.1
-0.2
carbon source
L-Lactate (C)
+0.1
-0.1
-0.7
-0.2
-0.0
carbon source
D-Lactate (C)
+0.2
-0.1
-0.8
-0.0
+0.1
carbon source
L-Glutamine (C)
-0.1
-0.1
-0.4
-0.2
+0.2
stress
Choline chloride 500 mM
-0.1
-0.0
-0.4
-0.3
+0.2
motility
outer cut, LB soft agar motility assay
-0.5
-0.6
+0.2
+0.1
+0.3
motility
outer cut, LB soft agar motility assay
-0.1
-0.3
+0.3
-0.2
-0.1
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.1
+0.2
-0.6
+0.1
+0.2
stress
KB-half, Pseudo5 supernatant
+0.0
+0.1
-0.4
+0.3
-0.1
stress
Thallium(I) acetate 0.008 mg/ml
+0.5
+0.1
-0.6
+0.1
+0.0
stress
1-ethyl-3-methylimidazolium acetate 10 mM
-0.2
+0.0
-0.2
+0.2
+0.3
stress
Cholin acetate 20 mM
+0.1
+0.1
-0.3
-0.0
+0.3
motility
outer cut, LB soft agar motility assay
+0.1
-0.2
-0.3
+0.6
+0.3
carbon source
L-Lysine (C)
-0.0
+0.2
-0.2
+0.2
+0.3
stress
perchlorate 75 mM
+0.1
+0.2
+0.5
+0.1
-0.3
stress
Ceftazidime 0.2 mg/ml
+0.0
-0.0
+0.9
-0.1
+0.0
anaerobic
Nitrate reduction with yeast extract
+0.2
+0.1
+0.6
+0.3
-0.2
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.1
+0.3
+0.5
+0.5
+0.4
stress
copper (II) chloride 2.5 mM
+0.7
+1.0
+1.3
+0.1
-0.1
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