Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_10035 and AO356_10040 are separated by 209 nucleotidesAO356_10040 and AO356_10045 overlap by 4 nucleotidesAO356_10045 and AO356_10050 overlap by 4 nucleotidesAO356_10050 and AO356_10055 overlap by 4 nucleotides AO356_10035: AO356_10035 - hypothetical protein, at 2,263,308 to 2,266,220 _10035 AO356_10040: AO356_10040 - dTDP-glucose 4,6-dehydratase, at 2,266,430 to 2,267,512 _10040 AO356_10045: AO356_10045 - glucose-1-phosphate thymidylyltransferase, at 2,267,509 to 2,268,384 _10045 AO356_10050: AO356_10050 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 2,268,381 to 2,268,926 _10050 AO356_10055: AO356_10055 - dTDP-4-dehydrorhamnose reductase, at 2,268,923 to 2,269,807 _10055
Group Condition AO356_10035 AO356_10040 AO356_10045 AO356_10050 AO356_10055
carbon source L-Histidine (C) -0.3 N.D. N.D. N.D. N.D.
carbon source D-Alanine (C) -0.2 N.D. N.D. N.D. N.D.
nitrogen source D-Serine (N) -0.2 N.D. N.D. N.D. N.D.
carbon source L-Alanine (C) -0.2 N.D. N.D. N.D. N.D.
stress Tetracycline 0.0001 mg/ml -0.2 N.D. N.D. N.D. N.D.
nitrogen source L-Isoleucine (N) -0.2 N.D. N.D. N.D. N.D.
carbon source Putrescine (C) -0.2 N.D. N.D. N.D. N.D.
stress Doxycycline hyclate 0.0001 mg/ml -0.2 N.D. N.D. N.D. N.D.
nitrogen source D-Alanine (N) -0.2 N.D. N.D. N.D. N.D.
nitrogen source L-Leucine (N) -0.2 N.D. N.D. N.D. N.D.
carbon source L-Histidine (C) -0.2 N.D. N.D. N.D. N.D.
stress Choline chloride 300 mM -0.1 N.D. N.D. N.D. N.D.
carbon source L-Lysine (C) -0.1 N.D. N.D. N.D. N.D.
carbon source L-Isoleucine (C) -0.1 N.D. N.D. N.D. N.D.
carbon source L-Proline (C) -0.1 N.D. N.D. N.D. N.D.
carbon source D-Trehalose (C) -0.1 N.D. N.D. N.D. N.D.
stress acetate 40 mM -0.1 N.D. N.D. N.D. N.D.
nitrogen source Gly-Glu (N) -0.1 N.D. N.D. N.D. N.D.
nitrogen source L-tyrosine (N) -0.1 N.D. N.D. N.D. N.D.
carbon source L-Lactate (C) -0.1 N.D. N.D. N.D. N.D.
carbon source L-Malic (C) -0.1 N.D. N.D. N.D. N.D.
carbon source D-Sorbitol (C) -0.1 N.D. N.D. N.D. N.D.
resistance 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine +0.1 N.D. N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay +0.1 N.D. N.D. N.D. N.D.
temperature LB 30C +0.1 N.D. N.D. N.D. N.D.
pH Minimal media pH7 +0.1 N.D. N.D. N.D. N.D.
carbon source D-Glucose (C) +0.1 N.D. N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay +0.2 N.D. N.D. N.D. N.D.
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine +0.2 N.D. N.D. N.D. N.D.
anaerobic Nitrate reduction with yeast extract +0.2 N.D. N.D. N.D. N.D.
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