Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_09740 and AO356_09745 are separated by 577 nucleotidesAO356_09745 and AO356_09750 are separated by 76 nucleotidesAO356_09750 and AO356_09755 are separated by 203 nucleotidesAO356_09755 and AO356_09760 are separated by 39 nucleotides AO356_09740: AO356_09740 - transposase, at 2,210,777 to 2,211,079 _09740 AO356_09745: AO356_09745 - hypothetical protein, at 2,211,657 to 2,212,019 _09745 AO356_09750: AO356_09750 - rRNA methylase, at 2,212,096 to 2,212,551 _09750 AO356_09755: AO356_09755 - preprotein translocase subunit SecB, at 2,212,755 to 2,213,237 _09755 AO356_09760: AO356_09760 - glutaredoxin, at 2,213,277 to 2,213,531 _09760
Group Condition AO356_09740 AO356_09745 AO356_09750 AO356_09755 AO356_09760
survival 4C survival; 360 hrs N.D. N.D. +0.1 -3.9 -0.8
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. N.D. +0.5 -4.0 -1.0
survival 4C survival; 1152 hrs N.D. N.D. +0.1 -3.2 -1.0
survival 4C survival; 192 hrs N.D. N.D. -0.0 -3.1 -0.8
survival 4C survival; 672 hrs N.D. N.D. -0.0 -3.2 -0.4
stress 1-ethyl-3-methylimidazolium lysine 10 mM N.D. N.D. -0.7 -1.3 -1.2
stress Choline chloride 500 mM N.D. N.D. +0.0 -2.3 -0.9
stress Choline chloride 300 mM N.D. N.D. +0.0 -2.3 -0.8
stress nitrate 200 mM N.D. N.D. -0.0 -2.2 -0.3
carbon source Carbon source Sucrose 2.5 mM N.D. N.D. -0.0 -1.5 -0.6
carbon source Carbon source Sucrose 5 mM N.D. N.D. +0.1 -0.8 -1.1
carbon source Carbon source Sucrose 5 mM N.D. N.D. -0.2 -1.1 -0.5
carbon source D-Trehalose (C) N.D. N.D. +0.1 -0.8 -1.0
stress Cholin acetate 40 mM N.D. N.D. +0.0 -0.8 -0.8
stress Chlorite 0.08 mM N.D. N.D. +0.1 -1.2 -0.3
stress copper (II) chloride 2.5 mM N.D. N.D. +0.4 -1.7 -0.0
nitrogen source D-Serine (N) N.D. N.D. +0.4 -0.1 -1.3
phage P5ss-5 phage 10-1 dilution SM calcl2 N.D. N.D. +0.5 -0.5 -1.0
anaerobic Nitrate reduction with yeast extract N.D. N.D. +0.5 +0.4 -1.0
motility outer cut, LB soft agar motility assay N.D. N.D. -0.4 +0.8 +0.9
stress perchlorate 75 mM N.D. N.D. -0.1 +0.4 +1.1
motility outer cut, LB soft agar motility assay N.D. N.D. +0.1 +0.6 +1.0
stress Cisplatin 0.0032 mg/ml N.D. N.D. +0.6 +0.5 +0.6
carbon source Fumarate (C) N.D. N.D. +0.5 +0.6 +0.7
stress Doxycycline hyclate 0.0001 mg/ml N.D. N.D. +0.2 +0.5 +1.1
stress Lomefloxacin 0.0005 mM N.D. N.D. -0.1 +0.6 +1.3
motility outer cut, LB soft agar motility assay N.D. N.D. -0.5 +1.0 +1.8
nitrogen source Adenosine (N) N.D. N.D. +0.4 +1.0 +1.0
stress methylglyoxal 0.016 vol% N.D. N.D. +0.1 +1.3 +1.4
stress Benzalkonium Chloride 0.016 mg/ml N.D. N.D. -0.2 +3.4 +2.5
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