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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_09635 and AO356_09640 overlap by 4 nucleotides
AO356_09640 and AO356_09645 are separated by 104 nucleotides
AO356_09645 and AO356_09650 are separated by 122 nucleotides
AO356_09650 and AO356_09655 are separated by 31 nucleotides
AO356_09635: AO356_09635 - histidine utilization regulatory protein hutD
(from data)
, at 2,183,367 to 2,183,936
_09635
AO356_09640: AO356_09640 - histidine utilization repressor, at 2,183,933 to 2,184,682
_09640
AO356_09645: AO356_09645 - Formiminoglutamic iminohydrolase (EC 3.5.3.13)
(from data)
, at 2,184,787 to 2,186,151
_09645
AO356_09650: AO356_09650 - lipocalin, at 2,186,274 to 2,186,843
_09650
AO356_09655: AO356_09655 - hypothetical protein, at 2,186,875 to 2,187,129
_09655
Group
Condition
AO356
_09635
AO356
_09640
AO356
_09645
AO356
_09650
AO356
_09655
carbon source
L-Histidine (C)
-4.7
+1.8
-4.5
-0.0
+0.0
carbon source
L-Histidine (C)
-2.6
+1.5
-4.2
+0.1
-0.1
nitrogen source
Inosine (N)
+0.1
-3.9
+0.1
+0.0
+0.2
carbon source
Inosine (C)
+0.0
-1.9
+0.0
+0.0
-0.2
carbon source
D-Lactate (C)
-0.1
-1.4
+0.1
+0.0
-0.3
nitrogen source
Gly-Glu (N)
+0.1
-1.4
-0.1
-0.0
-0.0
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.2
-2.0
+0.1
+0.3
+0.3
carbon source
D-Trehalose (C)
+0.0
-1.3
-0.0
+0.2
-0.3
motility
outer cut, LB soft agar motility assay
-0.9
+0.4
-0.4
-0.2
-0.2
carbon source
D-Fructose (C)
-0.1
-1.2
-0.1
-0.0
+0.1
resistance
20 mM putrescine
+0.2
-1.5
-0.1
+0.1
+0.1
nitrogen source
D-Serine (N)
+0.2
-1.3
+0.0
+0.1
-0.2
anaerobic
Nitrate reduction with yeast extract
-0.7
+0.0
-0.4
-0.1
+0.1
carbon source
L-Arginine (C)
-0.1
-1.0
+0.1
-0.0
-0.1
nitrogen source
Uridine (N)
+0.1
-1.4
-0.1
+0.1
+0.2
carbon source
L-Lysine (C)
+0.1
-1.0
-0.1
-0.1
+0.1
carbon source
L-Glutamine (C)
+0.1
-1.2
+0.0
+0.0
-0.1
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.1
-1.1
-0.2
+0.2
+0.3
carbon source
Carbon source D-Mannitol 10 mM
-0.4
-0.6
+0.1
+0.3
-0.3
carbon source
D,L-Lactate (C)
-0.1
-1.0
-0.0
+0.1
+0.1
nitrogen source
D-Glucosamine Hydrochloride (N)
+0.0
-0.9
-0.1
+0.1
+0.2
carbon source
D-Trehalose (C)
+0.2
-0.9
+0.1
+0.2
-0.2
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.0
-1.1
+0.1
+0.2
+0.1
carbon source
D-Lactate (C)
+0.1
-1.1
+0.0
+0.1
+0.3
carbon source
acetate (C)
+0.1
-0.8
-0.1
+0.0
+0.3
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.1
-0.8
-0.1
+0.1
+0.3
carbon source
L-Leucine (C)
+0.1
-0.7
-0.1
+0.3
+0.1
stress
Lomefloxacin 0.0005 mM
-0.4
-0.4
+0.2
+0.1
+0.2
survival
4C survival; 1152 hrs
-0.3
-0.1
+0.1
-0.5
+0.7
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.5
+0.3
+0.3
+0.2
+0.2
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