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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_09600 and AO356_09605 are separated by 129 nucleotides
AO356_09605 and AO356_09610 are separated by 171 nucleotides
AO356_09610 and AO356_09615 overlap by 4 nucleotides
AO356_09615 and AO356_09620 are separated by 71 nucleotides
AO356_09600: AO356_09600 - histidine ammonia-lyase, at 2,173,457 to 2,174,989
_09600
AO356_09605: AO356_09605 - Histidine ammonia-lyase (EC 4.3.1.3)
(from data)
, at 2,175,119 to 2,176,642
_09605
AO356_09610: AO356_09610 - ABC transporter for L-Histidine, ATPase component
(from data)
, at 2,176,814 to 2,177,644
_09610
AO356_09615: AO356_09615 - ABC transporter for L-Histidine, permease component
(from data)
, at 2,177,641 to 2,178,492
_09615
AO356_09620: AO356_09620 - ABC transporter for L-Histidine, periplasmic substrate-binding component
(from data)
, at 2,178,564 to 2,179,532
_09620
Group
Condition
AO356
_09600
AO356
_09605
AO356
_09610
AO356
_09615
AO356
_09620
carbon source
L-Histidine (C)
-4.8
-2.8
-5.1
-6.6
-3.7
carbon source
L-Histidine (C)
-4.7
-2.6
-4.7
-5.5
-4.4
stress
1-ethyl-3-methylimidazolium acetate 10 mM
-0.6
-0.1
-0.3
-0.4
-0.3
stress
Spectinomycin 0.0125 mg/ml
-1.2
-0.0
+0.2
-0.7
+0.1
stress
Nickel (II) chloride 0.625 mM
-0.9
-0.0
-0.2
-0.3
-0.1
stress
Tetracycline 0.0001 mg/ml
-0.5
+0.0
-0.2
-0.8
+0.1
stress
Nickel (II) chloride 0.625 mM
-0.3
-0.2
-0.4
-0.3
-0.2
carbon source
D-Gluconic Acid (C)
-0.4
-0.2
-0.1
-0.4
-0.1
anaerobic
Nitrate reduction with yeast extract
-1.1
-0.1
+0.2
+0.3
-0.3
pH
Minimal media pH9
+0.2
-0.2
-0.3
-0.4
-0.3
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.3
+0.3
-0.1
-0.4
-0.2
stress
Cholin acetate 40 mM
-0.5
-0.3
-0.3
+0.5
-0.1
survival
4C survival; 360 hrs
+0.5
-0.1
-0.4
-0.5
-0.1
carbon source
m-Inositol (C)
-0.7
-0.1
+0.3
-0.2
+0.1
stress
Fusidic 0.1 mg/ml
-0.9
+0.2
+0.0
-0.1
+0.2
carbon source
Uridine (C)
-0.7
-0.0
-0.1
+0.5
-0.0
stress
Aluminum chloride 5 mM
+0.1
+0.2
+0.2
-0.7
-0.0
stress
copper (II) chloride 2.5 mM
+0.7
+0.2
-0.2
-0.9
+0.1
stress
nitrate 200 mM
-0.6
+0.1
+0.6
-0.2
+0.0
stress
Lomefloxacin 0.0005 mM
+0.7
-0.1
+0.1
-0.6
-0.2
carbon source
Carnitine Hydrochloride (C)
+0.5
-0.3
-0.4
+0.2
-0.1
carbon source
Carbon source D-Mannitol 5 mM
+0.3
+0.2
+0.3
-0.6
-0.1
motility
outer cut, LB soft agar motility assay
+0.9
-0.2
-0.4
-0.2
-0.1
carbon source
L-Asparagine (C)
+0.3
-0.2
-0.2
+0.4
+0.1
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.6
+0.1
+0.2
+0.8
-0.1
carbon source
L-Alanine (C)
+0.6
-0.2
-0.0
+0.5
-0.1
carbon source
Carbon source D-Mannitol 2.5 mM
+0.5
-0.1
+0.1
+0.5
-0.1
carbon source
Carbon source Sucrose 5 mM
+0.3
-0.0
+0.4
+0.4
+0.2
carbon source
L-Lysine (C)
+0.6
-0.1
+0.4
+0.2
+0.3
stress
KB-half, Pseudo5 supernatant
+0.8
+0.3
+0.2
+0.4
+0.2
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