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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_09480 and AO356_09485 are separated by 60 nucleotides
AO356_09485 and AO356_09490 are separated by 207 nucleotides
AO356_09490 and AO356_09495 are separated by 10 nucleotides
AO356_09495 and AO356_09500 are separated by 266 nucleotides
AO356_09480: AO356_09480 - poly(R)-hydroxyalkanoic acid synthase, at 2,148,712 to 2,150,394
_09480
AO356_09485: AO356_09485 - TetR family transcriptional regulator, at 2,150,455 to 2,151,075
_09485
AO356_09490: AO356_09490 - poly(3-hydroxyalkanoate) granule-associated protein PhaF, at 2,151,283 to 2,152,194
_09490
AO356_09495: AO356_09495 - poly(3-hydroxyalkanoate) granule-associated protein PhaI, at 2,152,205 to 2,152,627
_09495
AO356_09500: AO356_09500 - polyhydroxyalkanoic acid system protein, at 2,152,894 to 2,153,169
_09500
Group
Condition
AO356
_09480
AO356
_09485
AO356
_09490
AO356
_09495
AO356
_09500
stress
nitrate 200 mM
-0.1
-0.3
-0.3
-0.2
-1.4
resistance
20 mM putrescine
-0.1
-0.1
-1.0
-0.2
-0.6
carbon source
acetate (C)
-0.2
+0.1
-1.2
-0.3
-0.3
temperature
LB 20C
+0.1
-0.2
-0.3
-0.2
-1.1
carbon source
L-Proline (C)
+0.1
-0.0
-1.1
+0.1
-0.6
nitrogen source
Urea (N)
-0.1
-0.1
-0.6
-0.5
-0.3
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.0
-0.2
-0.5
-0.3
-0.6
stress
KB-half, Pseudo5 supernatant
-0.2
-0.5
-1.2
-0.6
+1.0
nitrogen source
L-Phenylalanine (N)
-0.1
-0.2
-0.5
-0.7
+0.0
nitrogen source
D-Serine (N)
+0.2
+0.5
-1.0
-0.5
-0.7
nitrogen source
L-tyrosine (N)
-0.1
+0.2
-0.4
-0.4
-0.6
stress
Chlorite 0.08 mM
-0.1
-0.3
+0.3
-0.2
-0.9
stress
Spectinomycin 0.0125 mg/ml
+0.0
-0.5
+0.1
-0.3
-0.7
carbon source
Carnitine Hydrochloride (C)
+0.0
+0.0
-1.2
+0.0
+0.3
nitrogen source
Gly-Glu (N)
+0.1
+0.4
-0.6
-0.1
-0.3
stress
Sisomicin 0.001 mg/ml
-0.2
+0.1
+0.6
-0.6
-0.2
motility
outer cut, LB soft agar motility assay
+0.2
-0.1
-1.4
+0.7
+0.4
carbon source
L-Malic (C)
+0.0
+0.2
-0.7
+0.1
+0.6
carbon source
L-Lysine (C)
-0.1
+0.5
+0.7
-0.0
-0.9
anaerobic
Nitrate reduction with yeast extract
+0.5
+0.4
+0.3
-0.4
-0.6
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.3
-0.4
-0.3
-0.0
+0.9
motility
outer cut, LB soft agar motility assay
+0.2
+0.2
-0.4
+0.2
+0.5
carbon source
D-Trehalose (C)
+0.2
+0.3
+0.6
+0.5
-0.2
carbon source
D-Trehalose (C)
+0.1
+0.3
+0.4
+0.6
+0.1
carbon source
Putrescine (C)
+0.1
+0.2
+0.7
+0.2
+0.3
carbon source
Inosine (C)
+0.2
+0.3
+0.5
+0.2
+0.6
stress
copper (II) chloride 2.5 mM
+0.3
+0.6
+0.5
+0.4
+0.3
survival
4C survival; 1152 hrs
+0.2
+0.2
+1.9
-0.3
+0.4
carbon source
Ethanol (C)
+0.3
+0.3
+0.7
+0.8
+0.5
carbon source
Ethanol (C)
+0.2
+0.5
+0.7
+0.5
+0.7
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