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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_09390 and AO356_09395 are separated by 4 nucleotides
AO356_09395 and AO356_09400 overlap by 4 nucleotides
AO356_09400 and AO356_09405 overlap by 4 nucleotides
AO356_09405 and AO356_09410 overlap by 4 nucleotides
AO356_09390: AO356_09390 - shikimate kinase, at 2,126,794 to 2,127,312
_09390
AO356_09395: AO356_09395 - fimbrial protein, at 2,127,317 to 2,129,404
_09395
AO356_09400: AO356_09400 - pilus assembly protein PilP, at 2,129,401 to 2,129,949
_09400
AO356_09405: AO356_09405 - pilus assembly protein PilP, at 2,129,946 to 2,130,569
_09405
AO356_09410: AO356_09410 - pilus assembly protein PilN, at 2,130,566 to 2,131,132
_09410
Group
Condition
AO356
_09390
AO356
_09395
AO356
_09400
AO356
_09405
AO356
_09410
stress
Sisomicin 0.001 mg/ml
N.D.
-0.1
-0.8
-0.0
-0.5
phage
P5ss-5 phage 10-1 dilution SM calcl2
N.D.
-0.6
-0.5
-0.1
+0.3
nitrogen source
D-Serine (N)
N.D.
+0.1
-0.4
-0.4
-0.2
stress
copper (II) chloride 2.5 mM
N.D.
-0.6
-0.1
-0.4
+0.2
stress
nitrite 10 mM
N.D.
-0.6
-0.1
-0.1
-0.1
stress
1-ethyl-3-methylimidazolium chloride 200 mM
N.D.
-0.3
-0.3
-0.2
-0.0
stress
perchlorate 75 mM
N.D.
-0.1
-0.3
-0.2
-0.2
carbon source
Tween 20 (C)
N.D.
+0.0
-0.1
-0.1
-0.4
carbon source
Ethanol (C)
N.D.
+0.1
-0.1
-0.4
-0.1
anaerobic
Nitrate reduction with yeast extract
N.D.
-0.3
+0.2
+0.1
-0.5
carbon source
Glycerol (C)
N.D.
+0.2
-0.1
-0.1
-0.4
carbon source
D-Xylose (C)
N.D.
+0.0
-0.1
+0.2
-0.6
motility
outer cut, LB soft agar motility assay
N.D.
+0.4
-0.5
+0.0
-0.3
carbon source
L-Glutamic (C)
N.D.
+0.2
-0.2
-0.0
-0.4
carbon source
L-Lysine (C)
N.D.
+0.2
-0.1
-0.3
-0.1
stress
Cobalt chloride 0.08 mM
N.D.
-0.4
+0.2
+0.1
-0.2
stress
Chlorite 0.08 mM
N.D.
-0.3
+0.1
-0.1
+0.2
nitrogen source
Urea (N)
N.D.
+0.1
+0.2
-0.1
-0.3
stress
Chlorite 0.04 mM
N.D.
+0.3
-0.3
-0.2
+0.1
carbon source
D-Lactate (C)
N.D.
+0.2
+0.2
-0.2
-0.2
carbon source
L-Lysine (C)
N.D.
+0.4
-0.0
-0.0
-0.3
temperature
LB 37C
N.D.
+0.2
-0.1
-0.1
+0.3
motility
outer cut, LB soft agar motility assay
N.D.
+0.2
-0.2
+0.3
+0.2
carbon source
Carbon source D-Mannitol 10 mM
N.D.
+0.4
+0.2
+0.3
-0.3
stress
Nalidixic 0.004 mg/ml
N.D.
+0.0
+0.2
-0.0
+0.5
motility
outer cut, LB soft agar motility assay
N.D.
+0.7
+0.0
-0.2
+0.2
stress
Cobalt chloride 0.08 mM
N.D.
+0.1
+0.1
+0.3
+0.2
survival
4C survival; 1152 hrs
N.D.
-0.1
+0.1
+0.3
+0.4
stress
Doxycycline hyclate 0.0001 mg/ml
N.D.
+0.4
+0.3
+0.4
+0.2
stress
Rifampicin 0.0024 mg/ml
N.D.
+0.4
+0.8
+0.4
+0.2
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