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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_09345 and AO356_09350 are separated by 8 nucleotides
AO356_09350 and AO356_09355 overlap by 4 nucleotides
AO356_09355 and AO356_09360 are separated by 66 nucleotides
AO356_09360 and AO356_09365 are separated by 355 nucleotides
AO356_09345: AO356_09345 - type II secretion system protein GspD, at 2,111,468 to 2,113,393
_09345
AO356_09350: AO356_09350 - hypothetical protein, at 2,113,402 to 2,113,929
_09350
AO356_09355: AO356_09355 - cytochrome B, at 2,113,926 to 2,114,468
_09355
AO356_09360: AO356_09360 - multidrug transporter, at 2,114,535 to 2,116,001
_09360
AO356_09365: AO356_09365 - uroporphyrinogen decarboxylase, at 2,116,357 to 2,117,424
_09365
Group
Condition
AO356
_09345
AO356
_09350
AO356
_09355
AO356
_09360
AO356
_09365
stress
Sisomicin 0.001 mg/ml
+0.1
-0.4
-1.8
+0.2
N.D.
stress
methylglyoxal 0.016 vol%
-0.2
-0.5
-1.0
-0.1
N.D.
carbon source
Carbon source Sucrose 10 mM
+0.1
-1.1
-0.5
-0.0
N.D.
stress
Cisplatin 0.0032 mg/ml
-0.1
-0.9
-0.0
-0.3
N.D.
motility
outer cut, LB soft agar motility assay
+0.1
-0.0
-1.4
+0.0
N.D.
survival
4C survival; 1152 hrs
-0.2
-0.7
-0.3
-0.1
N.D.
stress
1-ethyl-3-methylimidazolium acetate 20 mM
-0.2
-0.6
-0.4
-0.1
N.D.
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.1
-1.4
+0.3
-0.3
N.D.
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.1
-0.8
-0.0
-0.2
N.D.
carbon source
Carbon source Sucrose 5 mM
-0.0
-0.8
-0.1
-0.1
N.D.
carbon source
Glycerol (C)
+0.0
-0.7
-0.3
-0.1
N.D.
lb
LB
+0.1
-0.5
-0.3
-0.1
N.D.
motility
outer cut, LB soft agar motility assay
-0.1
-0.3
-0.6
+0.2
N.D.
carbon source
L-Asparagine (C)
-0.1
-0.8
+0.1
+0.0
N.D.
stress
Benzalkonium Chloride 0.008 mg/ml
-0.1
-0.5
-0.3
+0.2
N.D.
carbon source
L-Aspartic Acid (C)
+0.1
-0.7
-0.1
-0.1
N.D.
carbon source
Carbon source D,L-Lactate 10 mM
+0.3
-0.4
-0.3
-0.2
N.D.
carbon source
D-Fructose (C)
-0.0
-0.7
+0.2
+0.0
N.D.
carbon source
Carbon source D-Mannitol 10 mM
+0.3
-0.4
-0.2
-0.1
N.D.
nitrogen source
Adenine (N)
-0.1
-0.7
+0.3
+0.1
N.D.
stress
Chlorite 0.04 mM
+0.2
-0.8
+0.1
+0.1
N.D.
pH
Minimal media pH8
+0.1
+0.3
-0.7
-0.0
N.D.
stress
nitrite 10 mM
-0.1
+0.5
-0.6
-0.0
N.D.
stress
KB-half, Pseudo5 supernatant
+0.2
-0.4
+0.3
-0.2
N.D.
carbon source
Carbon source D-Mannitol 10 mM
-0.1
-0.5
+0.4
+0.1
N.D.
stress
nitrate 200 mM
+0.0
+0.7
-0.9
+0.1
N.D.
motility
outer cut, LB soft agar motility assay
-0.1
+0.7
-0.3
-0.1
N.D.
stress
Vanillin 2.5 mM
+0.1
-0.4
+0.5
+0.1
N.D.
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.0
+0.7
-0.2
+0.3
N.D.
carbon source
D-Mannose (C)
-0.0
+0.6
+0.3
+0.2
N.D.
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