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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_08130 and AO356_08135 are separated by 164 nucleotides
AO356_08135 and AO356_08140 are separated by 276 nucleotides
AO356_08140 and AO356_08145 are separated by 3 nucleotides
AO356_08145 and AO356_08150 overlap by 4 nucleotides
AO356_08130: AO356_08130 - pyrophosphatase, at 1,819,220 to 1,819,525
_08130
AO356_08135: AO356_08135 - MFS transporter, at 1,819,690 to 1,819,983
_08135
AO356_08140: AO356_08140 - MATE family efflux transporter, at 1,820,260 to 1,821,609
_08140
AO356_08145: AO356_08145 - hypothetical protein, at 1,821,613 to 1,822,407
_08145
AO356_08150: AO356_08150 - type VI secretion protein, at 1,822,404 to 1,823,711
_08150
Group
Condition
AO356
_08130
AO356
_08135
AO356
_08140
AO356
_08145
AO356
_08150
nitrogen source
Gly-Glu (N)
-0.2
-1.3
-0.0
+0.0
-0.5
carbon source
Carbon source D-Mannitol 5 mM
-0.3
-0.5
-0.4
-0.1
+0.1
motility
outer cut, LB soft agar motility assay
-0.5
-0.6
+0.0
+0.4
-0.5
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.3
+0.5
-0.5
-0.8
-0.1
carbon source
Carbon source Sucrose 2.5 mM
-0.1
-0.9
-0.3
-0.0
+0.2
stress
Cobalt chloride 0.08 mM
+0.0
-1.1
-0.0
-0.3
+0.4
carbon source
Ethanol (C)
-0.4
+0.2
-0.3
-0.5
+0.0
carbon source
Carbon source D-Mannitol 5 mM
-0.4
-0.5
-0.3
+0.0
+0.2
carbon source
D-Glucose (C)
-0.2
-0.4
+0.2
-0.6
+0.1
anaerobic
Nitrate reduction with yeast extract
+0.1
-1.1
-0.3
+0.2
+0.1
stress
methylglyoxal 0.016 vol%
+0.3
-0.6
-0.0
-0.6
-0.0
carbon source
L-Leucine (C)
-0.5
-0.1
-0.1
+0.3
-0.4
pH
Minimal media pH7
+0.1
-0.8
+0.1
+0.2
-0.3
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.1
-1.3
+0.6
+0.2
-0.1
carbon source
L-Isoleucine (C)
-0.0
-0.4
+0.1
+0.3
-0.7
carbon source
succinate (C)
+0.1
-0.5
+0.2
-0.5
+0.3
carbon source
D-Lactate (C)
+0.2
-0.4
-0.3
-0.3
+0.3
nitrogen source
D-Serine (N)
+0.3
+0.2
-0.2
+0.0
-0.7
carbon source
D-Xylose (C)
+0.3
-0.6
+0.3
-0.5
+0.3
carbon source
Carbon source D,L-Lactate 10 mM
-0.0
-0.8
-0.1
+0.5
+0.2
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.4
-0.0
+0.2
-0.5
+0.6
stress
KB-half, Pseudo5 supernatant
-0.1
-0.4
+0.5
+0.4
-0.5
pH
Minimal media pH8
+0.4
-0.5
-0.2
+0.4
-0.2
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.8
+0.7
-0.0
+0.3
-0.1
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.5
+0.7
-0.1
+0.2
+0.0
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.4
+0.4
+0.0
+0.5
-0.2
motility
outer cut, LB soft agar motility assay
+0.2
+0.7
-0.1
-0.3
-0.2
stress
copper (II) chloride 2.5 mM
-0.2
+0.4
+0.6
-0.2
-0.3
carbon source
D-Fructose (C)
+0.2
-0.2
+0.3
-0.3
+0.5
carbon source
acetate (C)
+0.4
+0.1
-0.1
-0.4
+0.4
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