Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_07465 and AO356_07470 are separated by 10 nucleotidesAO356_07470 and AO356_07475 are separated by 28 nucleotidesAO356_07475 and AO356_07480 are separated by 2 nucleotidesAO356_07480 and AO356_07485 are separated by 105 nucleotides AO356_07465: AO356_07465 - RNA-binding protein, at 1,652,343 to 1,652,651 _07465 AO356_07470: AO356_07470 - MFS transporter, at 1,652,662 to 1,653,066 _07470 AO356_07475: AO356_07475 - transcription elongation factor GreA, at 1,653,095 to 1,653,571 _07475 AO356_07480: AO356_07480 - carbamoyl phosphate synthase large subunit, at 1,653,574 to 1,656,795 _07480 AO356_07485: AO356_07485 - carbamoyl phosphate synthase small subunit, at 1,656,901 to 1,658,037 _07485
Group Condition AO356_07465 AO356_07470 AO356_07475 AO356_07480 AO356_07485
pH Minimal media pH6 N.D. -0.3 N.D. -4.0 N.D.
temperature LB 20C N.D. +0.2 N.D. -4.4 N.D.
pH Minimal media pH8 N.D. -0.4 N.D. -3.5 N.D.
pH Minimal media pH6 N.D. +0.1 N.D. -4.0 N.D.
pH Minimal media pH7 N.D. -0.0 N.D. -3.7 N.D.
nitrogen source nitrite (N) N.D. +0.4 N.D. -3.9 N.D.
lb LB N.D. -0.3 N.D. -3.1 N.D.
nitrogen source Thymine (N) N.D. +0.1 N.D. -3.5 N.D.
stress Nalidixic 0.004 mg/ml N.D. -0.2 N.D. -3.1 N.D.
carbon source D-Sorbitol (C) N.D. -0.0 N.D. -3.1 N.D.
temperature LB 20C N.D. +0.4 N.D. -3.5 N.D.
carbon source D-Glucosamine Hydrochloride (C) N.D. -0.5 N.D. -2.6 N.D.
carbon source casaminos (C) N.D. -0.2 N.D. -2.9 N.D.
pH Minimal media pH7 N.D. -0.1 N.D. -2.9 N.D.
carbon source NAG (C) N.D. +0.2 N.D. -3.1 N.D.
carbon source L-Glutamine (C) N.D. -0.0 N.D. -2.9 N.D.
motility outer cut, LB soft agar motility assay N.D. -0.2 N.D. -2.7 N.D.
carbon source Ethanol (C) N.D. -0.2 N.D. -2.8 N.D.
pH Minimal media pH8 N.D. +0.2 N.D. -3.0 N.D.
survival 4C survival; 360 hrs N.D. +0.3 N.D. -3.0 N.D.
nitrogen source D-Alanine (N) N.D. +0.2 N.D. -2.8 N.D.
nitrogen source Adenosine (N) N.D. +0.2 N.D. -2.8 N.D.
nitrogen source L-Phenylalanine (N) N.D. +0.2 N.D. -2.8 N.D.
carbon source D-Ribose (C) N.D. +0.2 N.D. -2.7 N.D.
stress Spectinomycin 0.0125 mg/ml N.D. +0.5 N.D. -2.8 N.D.
pH Minimal media pH9 N.D. +0.5 N.D. -2.8 N.D.
carbon source L-Leucine (C) N.D. +0.4 N.D. -2.7 N.D.
stress Benzalkonium Chloride 0.016 mg/ml N.D. +0.5 N.D. -2.7 N.D.
carbon source m-Inositol (C) N.D. +0.4 N.D. -2.5 N.D.
carbon source acetate (C) N.D. +0.5 N.D. -2.5 N.D.
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